Development of indirect competitive ELISA for the detection of Salmonella typhimurium

Title
Development of indirect competitive ELISA for the detection of Salmonella typhimurium
Author(s)
김명희방재혁[방재혁]슈리티슈클라김영환[김영환]
Keywords
CHICKENS; KOREA; FOODBORNE; FOODS; SERUM; FEED
Issue Date
201204
Publisher
ARS DOCENDI
Citation
ROMANIAN BIOTECHNOLOGICAL LETTERS, v.17, no.2, pp.7194 - 7204
Abstract
Due to increased rate of Salmonella infections in humans and animals, a rapid indirect competitive enzyme-linked immunosorbent assay (IC-ELISA), was developed in this study for routine analytical detection of Salmonella typhimurium. Formalin killed whole cell lysate of S. typhimurium was subcutaneously injected as an antigen into rabbit to produce antibody. The optimum production of rabbit anti-S. typhimurium immunoglobulin G (IgG) was reached to the highest level on 8 week. A two step caprylic acid and ammonium sulfate precipitation method was used to purify rabbit anti-S. typhimurium IgG from the immunized rabbit sera. The purified IgG was used for the detection of S. typhimurium through IC-ELISA. The developed technique was able to detect 10(6) cells/ml of S. typhimurium. Also, the specificity of the proposed immunoassay was investigated with different foodborne pathogens including Escherichia coli O157:H7, Cronobacter muytjensii, Enterobacter aerogenes and Salmonella enteritidis which produced no significant reaction signal as compared with S. typhimurium. These findings indicate that IC-ELISA could be used as an alternative method for detection of S. typhimurium.
URI
http://hdl.handle.net/YU.REPOSITORY/28595
ISSN
1224-5984
Appears in Collections:
자연자원대학 > 식품공학과 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE