Ultra-high throughput detection of single cell beta-galactosidase activity in droplets using micro-optical lens array

Title
Ultra-high throughput detection of single cell beta-galactosidase activity in droplets using micro-optical lens array
Author(s)
임지석Jeremy Vrignon[Jeremy Vrignon]Philipp Gruner[Philipp Gruner]Christos Karamitros[Christos Karamitros]Manfred Konrad[Manfred Konrad]Jean-Christophe Baret[Jean-Christophe Baret]
Keywords
MICROFLUIDICS; SURFACTANTS; EMULSIONS
Issue Date
201311
Publisher
AMER INST PHYSICS
Citation
APPLIED PHYSICS LETTERS, v.103, no.20
Abstract
We demonstrate the use of a hybrid microfluidic-micro-optical system for the screening of enzymatic activity at the single cell level. Escherichia coli beta-galactosidase activity is revealed by a fluorogenic assay in 100 pl droplets. Individual droplets containing cells are screened by measuring their fluorescence signal using a high-speed camera. The measurement is parallelized over 100 channels equipped with microlenses and analyzed by image processing. A reinjection rate of 1 ml of emulsion per minute was reached corresponding to more than 10(5) droplets per second, an analytical throughput larger than those obtained using flow cytometry. (C) 2013 AIP Publishing LLC.
URI
http://hdl.handle.net/YU.REPOSITORY/28554http://dx.doi.org/10.1063/1.4830046
ISSN
0003-6951
Appears in Collections:
공과대학 > 기계공학부 > Articles
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