Protein detection through different platforms of immuno-loop-mediated isothermal amplification

Title
Protein detection through different platforms of immuno-loop-mediated isothermal amplification
Author(s)
주상우Mohammad Pourhassan-Moghaddam[Mohammad Pourhassan-Moghaddam]Mohammad Rahmati-Yamchi[Mohammad Rahmati-Yamchi]Abolfazl Akbarzadeh[Abolfazl Akbarzadeh]Hadis Daraee[Hadis Daraee]Kazem Nejati-Koshki[Kazem Nejati-Koshki]요네스피로즈살라리
Keywords
ROLLING-CIRCLE AMPLIFICATION; SEMICONDUCTOR QUANTUM DOTS; INFECTIOUS-DISEASES; ANTIGEN-DETECTION; VISUAL DETECTION; QUANTITATIVE DETECTION; SENSITIVE DETECTION; DNA AMPLIFICATION; GOLD NANOPROBES; NUCLEIC-ACIDS
Issue Date
201311
Publisher
SPRINGER
Citation
NANOSCALE RESEARCH LETTERS, v.8, pp.1 - 11
Abstract
Different immunoassay-based methods have been devised to detect protein targets. These methods have some challenges that make them inefficient for assaying ultra-low-amounted proteins. ELISA, iPCR, iRCA, and iNASBA are the common immunoassay-based methods of protein detection, each of which has specific and common technical challenges making it necessary to introduce a novel method in order to avoid their problems for detection of target proteins. Here we propose a new method nominated as 'immuno-loop-mediated isothermal amplification' or 'iLAMP'. This new method is free from the problems of the previous methods and has significant advantages over them. In this paper we also offer various configurations in order to improve the applicability of this method in real-world sample analyses. Important potential applications of this method are stated as well.
URI
http://hdl.handle.net/YU.REPOSITORY/28541http://dx.doi.org/10.1186/1556-276X-8-485
ISSN
1556-276X
Appears in Collections:
공과대학 > 기계공학부 > Articles
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