Quantitative determination and pattern recognition analyses of bioactive marker compounds from Dipsaci Radix by HPLC

Title
Quantitative determination and pattern recognition analyses of bioactive marker compounds from Dipsaci Radix by HPLC
Author(s)
손종근조병찬[조병찬]정수양[정수양]문동철[문동철]손건호[손건호]우미희[우미희]
Keywords
ASPER WALL; SAPONINS; ROOTS
Issue Date
201311
Publisher
PHARMACEUTICAL SOC KOREA
Citation
ARCHIVES OF PHARMACAL RESEARCH, v.36, no.11, pp.1345 - 1353
Abstract
In this study, quantitative and pattern recognition analyses were developed using HPLC/UV for the quality evaluation of Dipsaci Radix. For quantitative analysis, five major bioactive compounds were assessed. The separation conditions employed for HPLC/UV were optimized using ODS C-18 column (250 x 4.6 mm, 5 mu m) with a gradient of acetonitrile and water as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 212 nm. These methods were fully validated with respect to linearity, accuracy, precision, recovery, and robustness. The HPLC/UV method was applied successfully to the quantification of five major compounds in the extract of Dipsaci Radix. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of 17 Dipsaci Radix and four Phlomidis Radix samples. The results indicate that the established HPLC/UV method is suitable for quantitative analysis.
URI
http://hdl.handle.net/YU.REPOSITORY/28522http://dx.doi.org/10.1007/s12272-013-0162-y
ISSN
0253-6269
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약학대학 > 약학부 > Articles
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