Effect of porcine placenta steroid extract on myogenic satellite cell proliferation, transdifferentiation, and lipid accumulation

Title
Effect of porcine placenta steroid extract on myogenic satellite cell proliferation, transdifferentiation, and lipid accumulation
Author(s)
최인호이은주마지드라술캄리압둘루프바트무드바리스미리티[무드바리스미리티]이동목김상훈[김상훈]김태일[김태일]홍성구[홍성구]
Keywords
C/EBP-ALPHA; PPAR-GAMMA; GENE-EXPRESSION; MUSCLE-CELLS; DIFFERENTIATION; THIAZOLIDINEDIONES; ADIPOGENESIS; SERUM
Issue Date
201205
Publisher
SPRINGER
Citation
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, v.48, no.5, pp.326 - 333
Abstract
Intramuscular long-chain fatty acids (LCFAs) play an important role in energy production and initiation of mitochondrial oxidation of lipids. Herein, we report a natural porcine placenta steroid extract (PPSE) that stimulates transdifferentiation and lipid accumulation in bovine myogenic satellite cells (MSCs). The steroids hormones in PPSE were analyzed using enzyme-linked immunosorbant assay and presence of LCFA was established using gas chromatography. At 70 % confluent growth, cells were treated with PPSE, LCFAs, transdifferentiation cocktail and commercially available steroid hormones. The working concentrations of all chemicals were manipulated similar to PPSE. The cells were observed for morphological changes and subjected to quantitative analysis of lipid deposition on Days 2, 4, and 6 of treatment. PPSE-treated MSCs exclusively transformed into lipid-accumulated adipose-like cells (ALCs). However, myotubes or adipocytes were formed in cells treated with other chemicals. Expression of different genes was studied to ascertain the molecular mechanism involved in ALC formation. CD36, fatty acid binding protein 4, and peroxisome proliferator-activated receptor-gamma were up-regulated. The expression of CD36 was established through immunocyto-chemical analysis. A viability assay was used to confirm the effect of PPSE on proliferation of MSCs. Hence, a natural steroid extract from porcine was found as a nontoxic mixture, which induces lipid accumulation and transdifferentiation of MSCs to ALCs. From the gene expression studies, it was established that the extract works almost in homogenous manner with other lipid inducers.
URI
http://hdl.handle.net/YU.REPOSITORY/28373http://dx.doi.org/10.1007/s11626-012-9512-1
ISSN
1071-2690
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생명공학부 > 생명공학부 > Articles
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