Characterization of Urine-derived Cells From Upper Urinary Tract in Patients With Bladder Cancer

Title
Characterization of Urine-derived Cells From Upper Urinary Tract in Patients With Bladder Cancer
Author(s)
김현태전소영[전소영]이지선[이지선]김민정[김민정]김범수[김범수]김법완[김법완]권태균[권태균]
Keywords
MESENCHYMAL STEM-CELLS; BONE-MARROW; INCONTINENCE; DIFFERENTIATION; RECONSTRUCTION; UROTHELIUM; GENE
Issue Date
201205
Publisher
ELSEVIER SCIENCE INC
Citation
UROLOGY, v.79, no.5
Abstract
OBJECTIVE To investigate whether cells isolated from the upper urinary tract (UTCs) possess stem cell characteristics and could be an alternative cell source for patients with bladder cancer. Current tissue engineering approaches for urologic tissue regeneration require invasive tissue biopsies to obtain autologous cells, and these procedures are associated with potential complications, such as donor site morbidity. Recently, cells isolated from voided urine (VUCs) have been proposed as an alternative cell source for urologic tissue engineering. However, VUCs should not be used in patients with bladder cancer, because the voided urine sample could contain malignant cells. METHODS Urine samples were collected from the upper urinary tract of 4 male patients with bladder cancer using a ureteral catheter. The samples were centrifuged and the pellets plated for primary culture. The cells were analyzed for colony-forming unit, proliferation rate, cytogenetics, stem cell characterization, and tumorigenicity. The results were compared with those of VUCs collected from 3 healthy men. RESULTS The UTCs were able to form colonies, had a greater proliferation rate than the VUCs, and had a normal karyotype without any chromosomal aberrations. The UTCs possessed stem cell characteristics (expression of CD44+, CD73+, CD90+, CD105+, SSEA4+) and expressed several markers for urothelial, smooth muscle, and endothelial cell lineages. The UTCs did not form teratoma when implanted into the subcapsular space of a mouse kidney. CONCLUSION The UTCs possessed stem cell characteristics and can potentially be an alternative cell source for urologic tissue regeneration in patients with bladder cancer. UROLOGY 79: 1186.e1-1186.e7, 2012. (C) 2012 Elsevier Inc.
URI
http://hdl.handle.net/YU.REPOSITORY/28324http://dx.doi.org/10.1016/j.urology.2011.12.034
ISSN
0090-4295
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의과대학 > 비뇨기과학교실 > Articles
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