Molecularly Engineered Islet Cell Clusters for Diabetes Mellitus Treatment

Title
Molecularly Engineered Islet Cell Clusters for Diabetes Mellitus Treatment
Author(s)
육심명[육심명]정지헌정윤석[정윤석]홍성우[홍성우]임복현[임복현]서진원[서진원]박준범[박준범]이민형[이민형]안철희[안철희]이해신[이해신]이동윤[이동윤]변영로[변영로]
Keywords
INSULIN-SECRETION; PANCREATIC-ISLETS; TRANSPLANTATION; COMMUNICATION; ENCAPSULATION; APOPTOSIS; SURVIVAL; TERM
Issue Date
201205
Publisher
COGNIZANT COMMUNICATION CORP
Citation
CELL TRANSPLANTATION, v.21, no.8, pp.1775 - 1789
Abstract
Pancreatic islet transplantation is a promising method for curing diabetes mellitus. We proposed in this study a molecularly engineered islet cell clusters (ICCs) that could overcome problems posed by islet transplantation circumstances and host's immune reactions. A gene containing highly releasable exendin-4, an insulinotropic protein, was delivered into single islet cells to enhance glucose sensitivity; thereafter, the cells were reaggregated into small size ICCs. Then the surface of ICCs was modified with biocompatible poly(ethylene glycol)lipid (PEG) (C18) for preventing immune reactions. The regimen of ICCs with low doses of anti-CD154 mAb and tacrolimus could effectively maintain the normal glucose level in diabetic mice. This molecularly engineered PEG-Sp-Ex-4 ICC regimen prevented cell death in transplantation site, partly through improving the regulation of glucose metabolism and by preventing hypoxia- and immune response-induced apoptosis. Application of this remedy is also potentially far-reaching; one would be to help overcome islet supply shortage due to the limited availability of pancreas donors and reduce the immunosuppressant regimens to eliminate their adverse effects.
URI
http://hdl.handle.net/YU.REPOSITORY/28321http://dx.doi.org/10.3727/096368912X640628
ISSN
0963-6897
Appears in Collections:
약학대학 > 약학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE