Transcriptional analysis of hydroxycinnamoyl transferase (HCT) in various tissues of Hibiscus cannabinus in response to abiotic stress conditions

Title
Transcriptional analysis of hydroxycinnamoyl transferase (HCT) in various tissues of Hibiscus cannabinus in response to abiotic stress conditions
Author(s)
쵸드허리 임란[쵸드허리 임란]최보성[최보성]박상언[박상언]임현섭[임현섭]배한홍
Keywords
LIGNIN BIOSYNTHESIS; DOWN-REGULATION; PHENYLPROPANOID BIOSYNTHESIS; ARABIDOPSIS-THALIANA; BIOFUEL PRODUCTION; MOLECULAR-CLONING; COLD-ACCLIMATION; METHYL JASMONATE; THEOBROMA-CACAO; CELL-CULTURES
Issue Date
201205
Publisher
SOUTHERN CROSS PUBL
Citation
PLANT OMICS, v.5, no.3, pp.305 - 313
Abstract
We cloned a full-length gene from the kenaf plant putatively encoding hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase enzyme (HcHCT), which is involved in the lignin biosynthesis pathway. We examined the tissue and organ specific expression of an HcHCT ortholog during developmental stages and in response to abiotic stresses. The full-length of the HcHCT ortholog consisted of a 1,296 bp open reading frame (ORF) encoding 431 peptides. The molecular weight of deduced amino acids was 47.71 kDa, with an isoelectric point (pI) of 5.79. The deduced amino acid sequence showed 80-86% identities with HCTs of other plants. The deduced amino acid sequence of the HcHCT ortholog has a histidine containing motif (HHAAD), characteristic for acyl transfer catalysis. A second consensus sequence, a DFGWG block, is another acyl transferase of the BAHD family. Phylogenetic analysis showed the closest relationship (86%) with HCT of Populus trichodcarpa (ACC63882). According to quantitative real-time reverse transcription PCR (QPCR) analysis, HcHCT transcript was expressed in all the tissues and organs, but the highest expression was observed in roots and mature flowers. The expression of HcHCT transcript was also examined in stem tissues of 3-week-old kenaf plants in response to various abiotic stresses. The expression of HcHCT transcript was highly induced by all treatments, including wound, SA, NaCl, cold, H2O2, ABA, and drought. HcHCT was highly expressed in response to cold, SA, and H2O2 at 24 h, 6 h, and 6 h after treatment, respectively. Our results suggest that we have cloned the full-length gene putatively encoding for HCT, which is responsive to various abiotic stresses.
URI
http://hdl.handle.net/YU.REPOSITORY/28308
ISSN
1836-0661
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