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dc.contributor.authorChang-Ho Eun[Chang-Ho Eun]ko
dc.contributor.authorKyoko Takagi[Kyoko Takagi]ko
dc.contributor.author박경일ko
dc.contributor.authorMasahiko Maekawa[Masahiko Maekawa]ko
dc.contributor.authorShigeru Iida[Shigeru Iida]ko
dc.contributor.authorKazuo Tsugane[Kazuo Tsugane]ko
dc.date.accessioned2015-12-17T02:27:53Z-
dc.date.available2015-12-17T02:27:53Z-
dc.date.created2015-11-13-
dc.date.issued201205-
dc.identifier.citationPLANT AND CELL PHYSIOLOGY, v.53, no.5, pp.857 - 868-
dc.identifier.issn0032-0781-
dc.identifier.urihttp://hdl.handle.net/YU.REPOSITORY/28272-
dc.identifier.urihttp://dx.doi.org/10.1093/pcp/pcs060-
dc.description.abstractA large part of the rice genome is composed of transposons. Since active excision/reintegration of these mobile elements may result in harmful genetic changes, many transposons are maintained in a genetically or epigenetically inactivated state. However, some non-autonomous DNA transposons of the nDart1-3 subgroup, including nDart1-0, actively transpose in specific rice lines, such as pyl-v which carries an active autonomous element, aDart1-27, on chromosome 6. Although nDart1-3 subgroup elements show considerable sequence identity, they display different excision frequencies. The most active element, nDart1-0, had a low cytosine methylation status. The aDart1-27 sequence showed conservation between pyl-stb (pyl-v derivative line) and Nipponbare, which both lack autonomous activity for transposition of nDart1-3 subgroup elements. In pyl-v plants, the promoter region of the aDart1-27 transposase gene was more hypomethylated than in other rice lines. Treatment with the methylation inhibitor 5-azacytidine (5-azaC) induced transposition of nDart1-3 subgroup elements in both pyl-stb and Nipponbare plants; the new insertion sites were frequently located in genic regions. 5-AzaC treatment principally induced expression of Dart1-34 transposase rather than the other 38 aDart1-related elements in both pyl-stb and Nipponbare treatment groups. Our observations show that transposition of nDart1-3 subgroup elements in the nDart1/aDart1 tagging system is correlated with the level of DNA methylation. Our system does not cause somaclonal variation due to an absence of transformed plants, offers the possibility of large-scale screening in the field and can identify dominant mutants. We therefore propose that this tagging system provides a valuable addition to the tools available for rice functional genomics.-
dc.language영어-
dc.publisherOXFORD UNIV PRESS-
dc.subjectMETHYLATION-
dc.subjectARABIDOPSIS-
dc.subjectINACTIVATION-
dc.subjectELEMENTS-
dc.subjectPLANTS-
dc.subjectMAIZE-
dc.subjectGENE-
dc.subjectTRANSGENE-
dc.subjectRETROTRANSPOSITION-
dc.subjectMETHYLTRANSFERASE-
dc.titleActivation and Epigenetic Regulation of DNA Transposon nDart1 in Rice-
dc.typeArticle-
dc.identifier.wosid000304059900011-
dc.identifier.scopusid2-s2.0-84861056934-
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