Activation and Epigenetic Regulation of DNA Transposon nDart1 in Rice

Title
Activation and Epigenetic Regulation of DNA Transposon nDart1 in Rice
Author(s)
Chang-Ho Eun[Chang-Ho Eun]Kyoko Takagi[Kyoko Takagi]박경일Masahiko Maekawa[Masahiko Maekawa]Shigeru Iida[Shigeru Iida]Kazuo Tsugane[Kazuo Tsugane]
Keywords
METHYLATION; ARABIDOPSIS; INACTIVATION; ELEMENTS; PLANTS; MAIZE; GENE; TRANSGENE; RETROTRANSPOSITION; METHYLTRANSFERASE
Issue Date
201205
Publisher
OXFORD UNIV PRESS
Citation
PLANT AND CELL PHYSIOLOGY, v.53, no.5, pp.857 - 868
Abstract
A large part of the rice genome is composed of transposons. Since active excision/reintegration of these mobile elements may result in harmful genetic changes, many transposons are maintained in a genetically or epigenetically inactivated state. However, some non-autonomous DNA transposons of the nDart1-3 subgroup, including nDart1-0, actively transpose in specific rice lines, such as pyl-v which carries an active autonomous element, aDart1-27, on chromosome 6. Although nDart1-3 subgroup elements show considerable sequence identity, they display different excision frequencies. The most active element, nDart1-0, had a low cytosine methylation status. The aDart1-27 sequence showed conservation between pyl-stb (pyl-v derivative line) and Nipponbare, which both lack autonomous activity for transposition of nDart1-3 subgroup elements. In pyl-v plants, the promoter region of the aDart1-27 transposase gene was more hypomethylated than in other rice lines. Treatment with the methylation inhibitor 5-azacytidine (5-azaC) induced transposition of nDart1-3 subgroup elements in both pyl-stb and Nipponbare plants; the new insertion sites were frequently located in genic regions. 5-AzaC treatment principally induced expression of Dart1-34 transposase rather than the other 38 aDart1-related elements in both pyl-stb and Nipponbare treatment groups. Our observations show that transposition of nDart1-3 subgroup elements in the nDart1/aDart1 tagging system is correlated with the level of DNA methylation. Our system does not cause somaclonal variation due to an absence of transformed plants, offers the possibility of large-scale screening in the field and can identify dominant mutants. We therefore propose that this tagging system provides a valuable addition to the tools available for rice functional genomics.
URI
http://hdl.handle.net/YU.REPOSITORY/28272http://dx.doi.org/10.1093/pcp/pcs060
ISSN
0032-0781
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자연자원대학 > 원예생명과학과 > Articles
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