1-Bromopropane up-regulates cyclooxygenase-2 expression via NF-kappa B and C/EBP activation in murine macrophages

Title
1-Bromopropane up-regulates cyclooxygenase-2 expression via NF-kappa B and C/EBP activation in murine macrophages
Author(s)
한은희[한은희]양지혜[양지혜]김형균[김형균]최재호[최재호]틸락카날[틸락카날]민트롱도[민트롱도]정영철[정영철]이광열[이광열]정태천정혜광[정혜광]
Keywords
CCAAT/ENHANCER-BINDING PROTEIN; EPITHELIAL-CELLS; TRANSCRIPTION; PATHWAYS; CANCER; GENE; CARCINOGENESIS; INFLAMMATION; INVOLVEMENT; PI3K/AKT
Issue Date
201205
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
FOOD AND CHEMICAL TOXICOLOGY, v.50, no.5, pp.1616 - 1622
Abstract
1-Bromopropane (1-BP) has been used in industry as an alternative to ozone-depleting solvents. In the present study, we examined the effect of 1-BP on cyclooxygenase-2 (COX-2) gene expression and analyzed the molecular mechanism of its activity in murine RAW 264.7 macrophages. 1-BP dose-dependently increased COX-2 protein and mRNA levels, as well as COX-2 promoter-driven luciferase activity in macrophages. Additionally, exposure to 1-BP markedly enhanced the production of prostaglandin E-2 (PGE(2)), a major COX-2 metabolite, in macrophages. Transfection experiments with several human COX-2 promoter constructs revealed that 1-BP activated the transcription factors nuclear factor-kappa B (NF-kappa B) and CCAAT/enhancer-binding protein (C/EBP), but not AP-1 or the cyclic AMP response element binding protein. Furthermore, Akt and mitogen-activated protein (MAP) kinases were significantly activated by 1-BP. These results demonstrated that 1-BP induced COX-2 expression via NF-kappa B and C/EBP activation through the Akt/ERK and p38 MAP kinase pathways. These findings provide further insight into the signal transduction pathways involved in the inflammatory effects of 1-BP. (C) 2012 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/28267http://dx.doi.org/10.1016/j.fct.2012.02.006
ISSN
0278-6915
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약학대학 > 약학부 > Articles
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