Potent Whitening Activity of Aruncus dioicus Extract in B16F10 Melanoma Cell by Suppression of Melanin Biosynthesis

Title
Potent Whitening Activity of Aruncus dioicus Extract in B16F10 Melanoma Cell by Suppression of Melanin Biosynthesis
Author(s)
문용선김동희[김동희]박태순[박태순]황주영[황주영]손준호[손준호]
Keywords
TYROSINASE-RELATED PROTEIN-1; SKIN PIGMENTATION; MELANOGENESIS; TRANSCRIPTION; EXPRESSION; PATHWAY; TRP1
Issue Date
201312
Publisher
KOREAN SOC HORTICULTURAL SCIENCE
Citation
KOREAN JOURNAL OF HORTICULTURAL SCIENCE & TECHNOLOGY, v.31, no.6, pp.813 - 820
Abstract
Monoterpenoids were recently found as main biologically active compounds which is responsible for various physiological effect in goat's beard (Aruncus dioicus). Ethyl acetate extract of A. dioicus (AIDE) was treated to Bl6F10 melanoma cells for the examination of whitening activity. MTT assay was performed to evaluate cell toxicq and the result showed that slight cell toxicity (> 10%) by over 500 mu g-mL(-1). Thus, 0, 5, 10, or 50 mu g.mL(-1).: AIDE was used for further experiments. We found that tyrosinase activity was decreased according to ADE concentration, and the total melanin content was also dramatically reduced. Especially with 50 mu g.mL(-1) AIDE treatment tyrosinase activity was reduced to 35.6%, and 58.8% of melanin content was lowered. In addition, whitening related proteins including tyrosinase, tyrosinase related protein 1 (TRP1), TRP2, microphthalmia associated transcription factor (MITF) and cAMP and ix-otein kinase A (PKA) were reduced by AIDE treatment. It caused decreased phosphorylation of cAMP response binding protein (CREB) but increased phosphorylation of extracellular signal related kinase (ERK). Therefore, in this paper we would like to suggest the potent usage of A. dioicus natively grown in Ulleungdo, Korea as materials of functional cosmetics by confirming whitening activity related with melanin content.
URI
http://hdl.handle.net/YU.REPOSITORY/28245http://dx.doi.org/10.7235/hort.2013.13067
ISSN
1226-8763
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자연자원대학 > 원예생명과학과 > Articles
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