AltMV TGB1 Nucleolar Localization Requires Homologous Interaction and Correlates with Cell Wall Localization Associated with Cell-to-Cell Movement

Title
AltMV TGB1 Nucleolar Localization Requires Homologous Interaction and Correlates with Cell Wall Localization Associated with Cell-to-Cell Movement
Author(s)
배한홍남지륜[남지륜]남문[남문]이철호[이철호]이봉춘[이봉춘]John Hammond[John Hammond]임현섭[임현섭]
Keywords
TRIPLE-GENE-BLOCK; PLANT-VIRUS MOVEMENT; STRIPE-MOSAIC-VIRUS; SUBCELLULAR-LOCALIZATION; RNA-BINDING; TERMINAL REGION; PROTEIN; POTEXVIRUS; IDENTIFICATION; SUPPRESSION
Issue Date
201312
Publisher
KOREAN SOC PLANT PATHOLOGY
Citation
PLANT PATHOLOGY JOURNAL, v.29, no.4, pp.454 - 459
Abstract
The Potexvirus Alternanthera mosaic virus (ARMY) has multifunctional triple gene block (TGB) proteins, among which our studies have focused on the properties of the TGB1 protein. The TGB1 of ARMY has functions including RNA binding, RNA silencing suppression, and cell-to-cell movement, and is known to form homologous interactions. The helicase domains of ARMY TGB1 were separately mutated to identify which regions are involved in homologous TGB1 interactions. The yeast two hybrid system and Bimolecular Fluorescence Complementation (BiFC) in planta were utilized to examine homologous interactions of the mutants. Helicase motif I of AltMV TGB1 was found to be critical to maintain homologous interactions. Mutations in the remaining helicase motifs did not inhibit TGB1 homologous interactions. In the absence of homologous interaction of TGB1, subcellular localization of helicase domain I mutants showed distinctively different patterns from that of WT TGB1. These results provide important information to study viral movement and replication of ARMY.
URI
http://hdl.handle.net/YU.REPOSITORY/28195http://dx.doi.org/10.5423/PPJ.NT.04.2013.0045
ISSN
1598-2254
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생명공학부 > 생명공학부 > Articles
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