Genome Analysis of the Domestic Dog (Korean Jindo) by Massively Parallel Sequencing

Title
Genome Analysis of the Domestic Dog (Korean Jindo) by Massively Parallel Sequencing
Author(s)
김종주ryong nam Kim[ryong nam Kim]김대수[김대수]최상행[최상행]윤병하[윤병하]강아람[강아람]남성혁[남성혁]김동욱[김동욱]하지홍[하지홍]Atsushi Toyoda[Atsushi Toyoda]Asao Fujiyama[Asao Fujiyama]김애리[김애리]김민영[김민영]박근향[박근향]이강선[이강선]박홍석[박홍석]
Keywords
CONTROL REGION; CANINE; DNA; GENETICS; REPERTOIRES; DIVERSITY; DISEASE; BREEDS; TCOF1; AGE
Issue Date
201206
Publisher
OXFORD UNIV PRESS
Citation
DNA RESEARCH, v.19, no.3, pp.275 - 287
Abstract
Although pioneering sequencing projects have shed light on the boxer and poodle genomes, a number of challenges need to be met before the sequencing and annotation of the dog genome can be considered complete. Here, we present the DNA sequence of the undo dog genome, sequenced to 45-fold average coverage using Illumina massively parallel sequencing technology. A comparison of the sequence to the reference boxer genome led to the identification of 4 675 437 single nucleotide polymorphisms (SNPs, including 3 346 058 novel SNPs), 71 642 indels and 8131 structural variations. Of these, 339 non-synonymous SNPs and 3 indels are located within coding sequences (CDS). In particular, 3 non-synonymous SNPs and a 26-bp deletion occur in the TCOF1 locus, implying that the difference observed in cranial facial morphology between Jindo and boxer dogs might be influenced by those variations. Through the annotation of the undo olfactory receptor gene family, we found 2 unique olfactory receptor genes and 236 olfactory receptor genes harbouring non-synonymous homozygous SNPs that are likely to affect smelling capability. In addition, we determined the DNA sequence of the undo dog mitochondrial genome and identified undo dog-specific mtDNA genotypes. This undo genome data upgrade our understanding of dog genomic architecture and will be a very valuable resource for investigating not only dog genetics and genomics but also human and dog disease genetics and comparative genomics.
URI
http://hdl.handle.net/YU.REPOSITORY/28167http://dx.doi.org/10.1093/dnares/dss011
ISSN
1340-2838
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생명공학부 > 생명공학부 > Articles
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