Comparative Characteristics of Porous Bioceramics for an Osteogenic Response In Vitro and In Vivo

Title
Comparative Characteristics of Porous Bioceramics for an Osteogenic Response In Vitro and In Vivo
Author(s)
김석영Hye-Rim Lee[Hye-Rim Lee]Han-Jun Kim[Han-Jun Kim]Ji-Seung Ko[Ji-Seung Ko]Yong-Suk Choi[Yong-Suk Choi]Sun Hee Do[Sun Hee Do]안면환
Keywords
CALCIUM-PHOSPHATE CERAMICS; MATRIX METALLOPROTEINASE-13 PROMOTER; MESENCHYMAL STEM-CELLS; HYDROXYAPATITE CERAMICS; BONE-RESORPTION; PHENOTYPE EXPRESSION; ENDOTHELIAL-CELLS; RUBUS-COREANUS; DIFFERENTIATION; OSTEOBLASTS
Issue Date
201312
Publisher
PUBLIC LIBRARY SCIENCE
Citation
PLOS ONE, v.8, no.12
Abstract
Porous calcium phosphate ceramics are used in orthopedic and craniofacial applications to treat bone loss, or in dental applications to replace missing teeth. The implantation of these materials, however, does not induce stem cell differentiation, so suitable additional materials such as porous calcium phosphate discs are needed to influence physicochemical responses or structural changes. Rabbit adipose-derived stem cells (ADSC) and mouse osteoblastic cells (MC3T3-E1) were evaluated in vitro by the MTT assay, semi-quantitative RT-PCR, and immunoblotting using cells cultured in medium supplemented with extracts from bioceramics, including calcium metaphosphate (CMP), hydroxyapatite (HA) and collagen-grafted HA (HA-col). In vivo evaluation of the bone forming capacity of these bioceramics in rat models using femur defects and intramuscular implants for 12 weeks was performed. Histological analysis showed that newly formed stromal-rich tissues were observed in all the implanted regions and that the implants showed positive immunoreaction against type I collagen and alkaline phosphatase (ALP). The intramuscular implant region, in particular, showed strong positive immunoreactivity for both type I collagen and ALP, which was further confirmed by mRNA expression and immunoblotting results, indicating that each bioceramic material enhanced osteogenesis stimulation. These results support our hypothesis that smart bioceramics can induce osteoconduction and osteoinduction in vivo, although mature bone formation, including lacunae, osteocytes, and mineralization, was not prominent until 12 weeks after implantation.
URI
http://hdl.handle.net/YU.REPOSITORY/28166http://dx.doi.org/10.1371/journal.pone.0084272
ISSN
1932-6203
Appears in Collections:
공과대학 > 신소재공학부 > Articles
의과대학 > 정형외과학교실 > Articles
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