Characterization of the in vitro Activities of the P1 and Helper Component Proteases of Soybean mosaic virus Strain G2 and Tobacco vein mottling virus

Title
Characterization of the in vitro Activities of the P1 and Helper Component Proteases of Soybean mosaic virus Strain G2 and Tobacco vein mottling virus
Author(s)
임현섭[임현섭]장찬용[장찬용]남지륜[남지륜]리 메이지아 [리 메이지아 ]홍진성[홍진성]배한홍주호종[주호종]김홍기[김홍기]포드 리차드[포드 리차드]도마이어 레스[도마이어 레스]
Keywords
DIFFERENTIAL EXPRESSION; MULTIFUNCTIONAL PROTEIN; TRYPSIN-INHIBITORS; HC-PRO; POTYVIRUS; PLANT; KUNITZ
Issue Date
201206
Publisher
KOREAN SOC PLANT PATHOLOGY
Citation
PLANT PATHOLOGY JOURNAL, v.28, no.2, pp.197 - 201
Abstract
Potyviruses express their RNA genomes through the production of polyproteins that are processed in host cells by three virus-encoded proteases. Soybean plants produce large amounts of protease inhibitors during seed development and in response to wounding that could affect the activities of these proteases. The in vitro activities of two of the proteases of Soybean mosaic virus (SMV) and Tobacco vein mottling virus (TVMV) were compared in the rabbit reticulocyte lysate in vitro translation system using synthetic RNA transcripts. Transcripts produced from SMV and TVMV cDNAs that included the P1 and helper component-protease (HC-Pro) coding regions directed synthesis of protein products that were only partially processed. Unprocessed polyproteins were not detected from transcripts that included all of the P1, HC-Pro, P3 and portions of the cylindrical inclusion protein coding regions of either virus. Addition of soybean trypsin inhibitor to in vitro translation reactions increased the accumulation of the unprocessed polyprotein from TVMV transcripts, but did not alter the patterns of proteins produced from SMV. These experiments suggest that SMV- and TVMV-encoded proteases are differentially sensitive to protease inhibitors.
URI
http://hdl.handle.net/YU.REPOSITORY/28163http://dx.doi.org/10.5423/PPJ.OA.02.2012.0027
ISSN
1598-2254
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생명공학부 > 생명공학부 > Articles
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