Development of Rapid Assessment Method to Determine Bacterial Viability Based on Ultraviolet and Visible (UV-Vis) Spectroscopy Analysis Including Application to Bioaerosols

Title
Development of Rapid Assessment Method to Determine Bacterial Viability Based on Ultraviolet and Visible (UV-Vis) Spectroscopy Analysis Including Application to Bioaerosols
Author(s)
변정훈박철우[박철우]윤기영[윤기영]김경식[김경식]황정호[황정호]
Keywords
FLOW-CYTOMETRY; VEGETATIVE CELLS; FLUORESCENCE; SYSTEM; MICROORGANISMS; ENUMERATION; BIOMARKERS; EFFICIENCY; PARTICLES; RECOVERY
Issue Date
201206
Publisher
TAIWAN ASSOC AEROSOL RES-TAAR
Citation
AEROSOL AND AIR QUALITY RESEARCH, v.12, no.3, pp.399 - 408
Abstract
We evaluated a method for the assessment of bacterial viability that is based on ultraviolet and visible (UV-Vis) spectroscopy analysis. The quantities of intracellular materials inside a cell vary depending on change of bacterial viability by disruption of the membrane integrity. Therefore, normalized optical density in the range of 200-290 nm was analyzed to determine if it varied in samples containing different proportions of live bacteria. Our results indicate that samples containing higher proportions of live bacteria such as Escherichia coli, Bacillus subtilis, and Staphylococcus epidermidis had higher optical densities. In addition, the optical density at 230 nm divided by the optical density at 670 nm was found to have a strong linear correlation with bacterial viability (the R-2 values of E. coli, B. subtilis, and S. epidermidis are 0.9964, 0.9118, and 0.9861, respectively). Our proposed rapid assessment method takes less than three minutes and only requires optical measurements at 230 and 670 nm; therefore, it is simpler and faster than colony counting, fluorochromasia, or the dye-exclusion test. Moreover, our method was applied to bioaerosols, which are currently important issues in public health, microbiology, aerosol science, and other fields. In our study, the bacteria (E. coli) were dispersed into the air using a Collison-type atomizer, and were sampled in sterilized deionized water using an impinger with a pump. According to our method, the viability of E. coli was approximately 55.2%, which was similar to 52.5 +/- 4.7% determined from the LIVE/DEAD BacLight bacterial viability assay.
URI
http://hdl.handle.net/YU.REPOSITORY/28097http://dx.doi.org/10.4209/aaqr.2011.08.0129
ISSN
1680-8584
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공과대학 > 기계공학부 > Articles
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