Mass Production of Rubusoside Using a Novel Stevioside-Specific beta-Glucosidase from Aspergillus aculeatus

Title
Mass Production of Rubusoside Using a Novel Stevioside-Specific beta-Glucosidase from Aspergillus aculeatus
Author(s)
고진아[고진아]김영민[김영민]류영배[류영배]정형재[정형재]박태순[박태순]박수진[박수진]위영중김중수[김중수]김도만[김도만]이우송[이우송]
Keywords
GLUCOSYL ESTER; PURIFICATION; DERIVATIVES; PRINCIPLE
Issue Date
201206
Publisher
AMER CHEMICAL SOC
Citation
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.60, no.24, pp.6210 - 6216
Abstract
Rubusoside (R) is a natural sweetener and a solubilizing agent with antiangiogenic and antiallergic properties. However, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable R-production method. A stevioside (ST)-specific beta-glucosidase (SSGase) was selected and purified 7-fold from Aspergillus aculeatus Viscozyme L by a two-step column chromatography procedure. The 79 kDa protein was stable from pH 3.0 to pH 7.0 at 50-60 degrees C. Hydrolysis of ST by SSGase produced R and steviol monoglucosyl ester as determined by H-1 and C-13 nuclear magnetic resonance (NMR). Importantly, SSGase showed higher activity toward ST than other beta-linked glucobioses. The optimal conditions for R production were 280 mM ST and 16.6 mu L of SSGase at pH 5.1 and 63 degrees C. This is the first discussion detailing the production of R by enzymatic hydrolysis of ST and is useful for the food additive and pharmaceutical industries.
URI
http://hdl.handle.net/YU.REPOSITORY/28075http://dx.doi.org/10.1021/jf300531e
ISSN
0021-8561
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자연자원대학 > 식품공학과 > Articles
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