Discrimination of Phellodendron amurense and P-chinense Based on DNA Analysis and the Simultaneous Analysis of Alkaloids

Title
Discrimination of Phellodendron amurense and P-chinense Based on DNA Analysis and the Simultaneous Analysis of Alkaloids
Author(s)
손종근육진아[육진아]정명선이미영[이미영]서창섭이응이승호문동철[문동철]이혜원[이혜원]이제현[이제현]박주영[박주영]고병섭[고병섭]
Keywords
CELLULAR IMMUNE-RESPONSE; BARK; PRINCIPLE; SUPPRESS; EXTRACT; CORTEX; PCR
Issue Date
201206
Publisher
PHARMACEUTICAL SOC KOREA
Citation
ARCHIVES OF PHARMACAL RESEARCH, v.35, no.6, pp.1045 - 1054
Abstract
Phellodendri Cortex is the bark of the stems of Phellodendron amurense Ruprecht or P. chinense Schneider (Rutaceae), which is orginated from periderm. The internal transcribed spacer sequences of 20 originated plants and identified samples were analyzed. The result showed that the 99% of the base sequences of P. amurense were identical to that of P. chinense, but the differentiation of P. amurense and P. chinense was difficult. In addition, the ribulose-1, 5-bisphospate carboxylase large subunit (rbcL) intergenic spacer sequences of specific parts produced the same result. However, when the analysis was carried out by using the RAPD (randomly amplification polymorphism DNA) analysis method, which utilizes 48 randomly primers, it allowed us to confirm the polymorphism of P. amurense and P. chinense in 12 primers. A high-performance liquid chromatographic (HPLC) method was developed and validated for the simultaneous quantitation of berberine, palmatine and jatrorrhizine in a traditional herbal drug, Phellodendri Cortex. The HPLC method was applied successfully to the quantification of three constituents in the extract of twenty Phellodendri Cortex. The results indicated that the established HPLC and RAPD methods are suitable for the quantitative analysis and the quality control multi-simultaneous discrimination in Phellodendri Cortex.
URI
http://hdl.handle.net/YU.REPOSITORY/28063http://dx.doi.org/10.1007/s12272-012-0612-y
ISSN
0253-6269
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약학대학 > 약학부 > Articles
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