Molecular cloning and expression analysis of pig CD138

Title
Molecular cloning and expression analysis of pig CD138
Author(s)
최인호김지은배준범[배준범]정선아[정선아]이주연[이주연]이현정[이현정]최봉환[최봉환]전태훈[전태훈]
Keywords
HEPARAN-SULFATE PROTEOGLYCANS; SYNDECAN-1; DISEASE; GROWTH; FAMILY
Issue Date
201312
Publisher
ELSEVIER SCI LTD
Citation
RESEARCH IN VETERINARY SCIENCE, v.95, no.3, pp.1021 - 1025
Abstract
CD138 (syndecan-1) interacts with various components of the extracellular matrix and associates with the actin cytoskeleton. In this study, we cloned pig CD138 cDNA and determined its complete cDNA sequence. Pig CD138 cDNA contained an open reading frame (930 bp) encoding 309 amino acids with five well conserved putative glycosaminoglycan attachment sites, a putative cleavage site for matrix metalloproteinases, and conserved motifs involved in signal transduction among mammalian species. Pig CD138 mRNA was detected in various tissues, including lymphoid and non-lymphoid organs, indicating the multicellular functions of CD138 in pigs. Western blot and flow cytometry analyses detected an approximate 35 kDa pig CD138 protein expressed on the cell surface. Further immunohistochemistry analysis revealed that CD138 expression was mainly observed in submucosa and lamina propria of the pig small intestine. Further study will be necessary to define the functional importance of CD138 during specific infectious diseases in pigs. (C) 2013 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/28049http://dx.doi.org/10.1016/j.rvsc.2013.09.010
ISSN
0034-5288
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생명공학부 > 생명공학부 > Articles
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