Biomineralization-based conversion of carbon dioxide to calcium carbonate using recombinant carbonic anhydrase

Title
Biomineralization-based conversion of carbon dioxide to calcium carbonate using recombinant carbonic anhydrase
Author(s)
김창섭김임규[김임규]조병훈[조병훈]강동균[강동균]최유성[최유성]차형준[차형준]
Keywords
NEISSERIA-GONORRHOEAE; ESCHERICHIA-COLI; CO2; TRANSFORMATION; PURIFICATION; POLYMORPHS; MECHANISM; GROWTH; CRYSTALLIZATION; MORPHOLOGY
Issue Date
201206
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
CHEMOSPHERE, v.87, no.10, pp.1091 - 1096
Abstract
Recently, as a mimic of the natural biomineralization process, the use of carbonic anhydrase (CA), which is an enzyme catalyzing fast reversible hydration of carbon dioxide to bicarbonate, has been suggested for biological conversion of CO2 to valuable chemicals. While purified bovine CA (BCA) has been used in previous studies, its practical utilization in CO2 conversion has been limited due to the expense of BCA preparation. In the present work, we investigated conversion of CO2 into calcium carbonate as a target carbonate mineral by using a more economical, recombinant CA. To our knowledge, this is the first report of the usage of recombinant CA for biological CO2 conversion. Recombinant a-type CA originating in Neisseria gonorrhoeae (NCA) was highly expressed as a soluble form in Escherichia coli. We found that purified recombinant NCA which showed comparable CO2 hydration activity to commercial BCA significantly promoted formation of solid CaCO3 through the acceleration of CO2 hydration rate, which is naturally slow. In addition, the rate of calcite crystal formation was also accelerated using recombinant NCA. Moreover, non-purified crude recombinant NCA also showed relatively significant ability. Therefore, recombinant CA could be an effective, economical biocatalyst in practical CO2 conversion system. (C) 2012 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/28019http://dx.doi.org/10.1016/j.chemosphere.2012.02.003
ISSN
0045-6535
Appears in Collections:
이과대학 > 화학생화학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE