Effect of Gender-Specific Adult Bovine Serum on Gene Expression During Myogenesis

Title
Effect of Gender-Specific Adult Bovine Serum on Gene Expression During Myogenesis
Author(s)
최인호이은주무드바리스미리티[무드바리스미리티]김지회남상섭[남상섭]
Keywords
Serum; Sex Steroid; Myogenesis; Muscle
Issue Date
201206
Publisher
한국동물자원과학회
Citation
한국동물자원과학회지, v.54, no.3, pp.219 - 226
Abstract
Gender specificity in muscle growth and development is well known. Genesis of muscle is dependent on proliferation and differentiation potential of resident myogenic satellite cells(MSCs) present in muscle fibers. Multipotential capacity of forming myocyte, osteocyte, and adipocyte like cell makes MSCs a unique stem cell. To understand the molecular mechanism involved in determination of muscle quality due to difference in hormone concentration of different gender of animals, MSCs were isolated from bovine skeletal muscle and cultured in male, female, and castrated serum supplemented media. DNA microarray used consisted of 24,000 spots with 70 mer oligo in each spot. A total of 88 genes were up-regulated and 551 genes were down-regulated by more than two fold. Among up-regulated gene, 33, 34, and 21 genes were found up-regulated in cells grown in male, female, and castrated serum, respectively. Interestingly, male serum showed 4, female 11 and castrated male showed 4 genes expressed highly in each gender. Further study on the highly up-regulated gene may unfold the mystery of gender specificity found in muscle development. Also, the identification of differentially expressed genes in gender-specific serum will add information on infrastructure of bovine genome research.
URI
http://hdl.handle.net/YU.REPOSITORY/27964http://dx.doi.org/10.5187/JAST.2012.54.3.219
ISSN
1598-9429
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생명공학부 > 생명공학부 > Articles
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