Grape skin extract reduces adipogenesis- and lipogenesis-related gene expression in 3T3-L1 adipocytes through the peroxisome proliferator-activated receptor-gamma signaling pathway

Title
Grape skin extract reduces adipogenesis- and lipogenesis-related gene expression in 3T3-L1 adipocytes through the peroxisome proliferator-activated receptor-gamma signaling pathway
Author(s)
조경현정유석홍주헌[홍주헌]정희경[정희경]
Keywords
SALT-INDUCIBLE KINASE; ADIPOSE-TISSUE; PPAR-GAMMA; LIPID-ACCUMULATION; DIFFERENTIATION; CELLS; PHOSPHORYLATION; STEROIDOGENESIS; RESVERATROL; ENZYMES
Issue Date
201207
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
NUTRITION RESEARCH, v.32, no.7, pp.514 - 521
Abstract
We previously reported that grape skin ethanol extract (GSE) decreases adipogenic transcription factor gene expression, inhibiting triglyceride accumulation in 3T3-L1 adipocytes. In this study, we hypothesized that GSE may induce differential expression profiles in adipocytes, thus providing protection against obesity. Thirty-five genes involved in the peroxisome proliferator-activated receptor-gamma (PPAR gamma) signaling pathway, lipid metabolism, or adipogenesis were identified through microarray analysis of adipocytes treated with GSE. Expression of the genes involved in PPAR gamma signaling, Adipoq, Scd1, Nr1h3, Fabp5, Scd2, and Pparg decreased with GSE treatment, whereas expression of Ppargc1a increased. Lipid metabolism-associated genes Mlxp1, Stat5a, Hsl, Plin1, and Vdr were down-regulated. Interestingly, GSE also affected expression of genes related to the mitogen-activated protein kinases pathway. GSE extract treatment decreased expression of aP2, Fas, and Tnfa, known markers of adipogenesis, as measured by real-time polymerase reaction. These findings demonstrate the antiadipogenic effects of GSE on 3T3-L1 adipocytes at the genetic level, primarily on the PPAR gamma signaling pathway. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/27698http://dx.doi.org/10.1016/j.nutres.2012.06.001
ISSN
0271-5317
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생명공학부 > 생명공학부 > Articles
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