A cell electrofusion microfluidic chip using discrete coplanar vertical sidewall microelectrodes

Title
A cell electrofusion microfluidic chip using discrete coplanar vertical sidewall microelectrodes
Author(s)
주상우닝후Jun Yang[Jun Yang]스즈첸Xiaolin Zhang[Xiaolin Zhang]Xiaolin Zheng[Xiaolin Zheng]
Keywords
EMBRYONIC STEM-CELLS; SOMATIC-CELLS; MAMMALIAN-CELLS; MESOPHYLL CELL; FUSION; DEVICE; DIELECTROPHORESIS; PROTOPLASTS; HYBRIDS
Issue Date
201207
Publisher
WILEY-BLACKWELL
Citation
ELECTROPHORESIS, v.33, no.13, pp.1980 - 1986
Abstract
A novel cell electrofusion microfluidic chip using discrete coplanar vertical sidewall electrodes has been designed, fabricated, and tested. The device contains a serpentine-shaped microchannel with 22 500 pairs of vertical sidewall microelectrodes patterned on two opposing vertical sidewalls of the microchannel. The adjacent microelectrodes on each sidewall are separated by coplanar SiO2-Polysilicon-SiO2/silicon. This design of coplanar discrete vertical sidewall electrodes eliminates the dead area present in previous designs using continuous three-dimensional (3D) protruding sidewall electrodes, and generates uniform electric field along the height of the microchannel, leading to a lower voltage required for cell fusion compared to designs using 2D thin-film electrodes. This device is tested to fuse NIH3T3 cells under a low voltage (similar to 9 V). Almost 100% cells are aligned to the edge of the discrete microelectrodes, and cell-cell pairing efficiency reaches 70%. The electrofusion efficiency is above 40% of the total cells loaded into the device, which is much higher than traditional fusion methods and existing microfluidic devices using continuous 3D protruding sidewall microelectrodes.
URI
http://hdl.handle.net/YU.REPOSITORY/27674http://dx.doi.org/10.1002/elps.201100579
ISSN
0173-0835
Appears in Collections:
공과대학 > 기계공학부 > Articles
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