Agrobacterium rhizogenes-mediated genetic transformation of radish (Raphanus sativus L. cv. Valentine) for accumulation of anthocyanin

Title
Agrobacterium rhizogenes-mediated genetic transformation of radish (Raphanus sativus L. cv. Valentine) for accumulation of anthocyanin
Author(s)
배한홍김연복[김연복]박남일[박남일]김행훈[김행훈]김영선[김영선]이미영[이미영]박상언[박상언]
Keywords
HAIRY ROOT; RED RADISH; CULTURES; SYSTEMS
Issue Date
201207
Publisher
SOUTHERN CROSS PUBL
Citation
PLANT OMICS, v.5, no.4, pp.381 - 385
Abstract
An efficient protocol for the transformation of radish (Raphanus sativus L. cv. Valentine) hairy root cultures was initiated using cotyledon explants and infected by Agrobacterium rhizogenes R1000, a strain with the binary vector pBI121. Kanamycin-resistant roots appeared on explants at 4 weeks after infection with A. rhizogenes and these roots were maintained on hormone-free selection medium. PCR analysis of the neomycin phosphotransferase (NTPII) gene confirmed transformation in 15 plants of kanamycin-resistant hairy root cultures from a total 20 plants. Detection of high levels of beta-glucuronidase (GUS) transcripts and enzyme activity, as well as GUS histochemical localization, also confirmed the stable genetic transformation. We inoculated isolated hairy roots in liquid medium to promote rapid growth and production of anthocyanin. Among the different auxin treatments, 1.0 mg/L 2,4-D treatment resulted the highest production of anthocyanin (30.9 mg/L) that was 26 times higher as compared to control (1.2 mg/L). Transgenic root cultures of R. sativus L. cv. Valentine will allow investigation of the molecular and metabolic regulation of anthocyanin biosynthesis and evaluation of the genetic engineering potential of this species.
URI
http://hdl.handle.net/YU.REPOSITORY/27607
ISSN
1836-0661
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생명공학부 > 생명공학부 > Articles
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