Inula japonica extract inhibits mast cell-mediated allergic reaction and mast cell activation

Title
Inula japonica extract inhibits mast cell-mediated allergic reaction and mast cell activation
Author(s)
장현욱유예이응김미화양주혜조광선박효현[박효현]박영나손종근이은경[이은경]이선
Keywords
NF-KAPPA-B; ARACHIDONIC-ACID; TYROSINE KINASE; PHOSPHOINOSITIDE 3-KINASE; SIGNAL-TRANSDUCTION; INFLAMMATION; EXPRESSION; PROTEIN; KIT; RECEPTOR
Issue Date
201208
Publisher
ELSEVIER IRELAND LTD
Citation
JOURNAL OF ETHNOPHARMACOLOGY, v.143, no.1, pp.151 - 157
Abstract
Ethnopharmacological relevance: The flowers of Inula japonica (Inulae Flos) have long been used in traditional medicine for the treatment of bronchitis, digestive disorders, and inflammation. However, the mechanisms underlying its anti-inflammatory effects remain yet to be elucidated. The objectives of this study were 1) to assess the anti-allergic activity of the ethanol extract of flowers of Inula japonica extract (IFE) in vivo, 2) to investigate the mechanism of its action on mast cells in vitro, and 3) to identify its major phytochemical compositions. Materials and methods: The anti-allergic activity of IFE was evaluated using mouse bone marrow-derived mast cells (BMMCs) in vitro and a passive cutaneous anaphylaxis (PCA) animal model in vivo. The effects of IFE on mast cell activation were evaluated in terms of degranulation, eicosanoid generation, Ca2+ influx, and immunoblotting of various signaling molecules. Results: IFE inhibited degranulation and the generation of eicosanoids (PGD(2) and LTC4) in stem cell factor (SCF)-stimulated BMMCs. Biochemical analysis of the SCF-mediated signaling pathways demonstrated that IFE inhibited the activation of multiple downstream signaling processes including mobilization of intracellular Ca2+ and phosphorylation of the mitogen-activated protein kinases (MAPKs). PLC gamma 1, and cPLA(2) pathways. When administered orally, IFE attenuated the mast cell-mediated PCA reaction in IgE-sensitized mice. Its major phytochemical composition included three sesquiter-penes, 1-O-acetylbritannilactone, britanin and tomentosin. Conclusions: This study suggests that IFE modulates eicosanoids generation and degranulation through the suppression of SCF-mediated signaling pathways that would be beneficial for the prevention of allergic inflammatory diseases. Anti-allergic activity of IFE may be in part attributed particularly to the presence of britanin and tomentosin as major components evidenced by a HPLC analysis. Crown Copyright (C) 2012 Published by Elsevier Ireland Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/27562http://dx.doi.org/10.1016/j.jep.2012.06.015
ISSN
0378-8741
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약학대학 > 약학부 > Articles
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