Anti-obesity effect of resveratrol-amplified grape skin extracts on 3T3-L1 adipocytes differentiation

Title
Anti-obesity effect of resveratrol-amplified grape skin extracts on 3T3-L1 adipocytes differentiation
Author(s)
서정숙장선화Bo Huang[Bo Huang]최수경
Keywords
ACTIVATED RECEPTOR-GAMMA; STEAROYL-COA DESATURASE-1; PPAR-GAMMA; ADIPOSE-TISSUE; TRANSCRIPTION FACTOR; INSULIN-SENSITIVITY; GENE-EXPRESSION; TRANSGENIC MICE; BINDING PROTEIN; C/EBP-ALPHA
Issue Date
201208
Publisher
KOREAN NUTRITION SOC
Citation
NUTRITION RESEARCH AND PRACTICE, v.6, no.4, pp.286 - 293
Abstract
Resveratrol (3,4,5-trihydroxy-trans-stilbene), a phytoalexin found in grape skin, grape products, and peanuts as well as red wine, has been reported to have various biological and pharmacological properties. The purpose of this study was to investigate the anti-obesity effect of resveratrol-amplified grape skin extracts on adipocytes. The anti-obesity effects of grape skin extracts were investigated by measuring proliferation and differentiation in 3T3-L1 cells. The effect of grape skin ethanol extracts on cell proliferation was detected by the MTS assay. The morphological changes and degree of adipogenesis of preadipocyte 3T3-L1 cells were measured by Oil Red-O staining assay. Treatment with extracts of resveratrol-amplified grape skin decreased lipid accumulation and glycerol-3-phosphate dehydrogenase activity without affecting 3T3-L1 cell viability. Grape skin extract treatment resulted in significantly attenuated expression of key adipogenic transcription factors, including peroxisome proliferator-activated receptor, CCAAT/enhancer-binding proteins, and their target genes (FAS, aP2, SCD-1, and LPL). These results indicate that resveratrol-amplified grape skin extracts may be useful for preventing obesity by regulating lipid metabolism.
URI
http://hdl.handle.net/YU.REPOSITORY/27528http://dx.doi.org/10.4162/nrp.2012.6.4.286
ISSN
1976-1457
Appears in Collections:
생활과학대학 > 식품영양학과 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE