Evaluation and biosynthetic incorporation of chlorotyrosine into recombinant proteins

Title
Evaluation and biosynthetic incorporation of chlorotyrosine into recombinant proteins
Author(s)
윤형돈니라이클람아야두라이[니라이클람아야두라이]카나가벨디판쿠마나다라잔사라바난부디사[부디사]
Keywords
TRANSFER-RNA SYNTHETASE; GREEN FLUORESCENT PROTEIN; AMINO-ACID INCORPORATIONS; IN-VIVO; TRYPTOPHAN ANALOGS; 3-CHLOROTYROSINE; MYELOPEROXIDASE; TYROSINE; MARKER; SITE
Issue Date
201208
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Citation
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.17, no.4, pp.679 - 686
Abstract
Recently, non-canonical amino acids (NCAA) incorporation was developed to enhance the functional properties of proteins. Incorporation of NCAA containing chlorine atom is conceptually an attractive approach to prepare pharmacologically active substances, which is a difficult task since chlorine is bulky atom. In this study, we evaluated the efficiency and extent of in vivo incorporation of tyrosine analogue 3-chlorotyrosine [(3-Cl)Tyr] into the recombinant proteins GFP and GFPHS (highly stable GFP). The incorporation of (3-Cl)Tyr into GFP leads to dramatic reduction in the expression level of protein. On the other hand, the incorporation of (3-Cl)Tyr into GFPHS was expressed well as a soluble form. In addition we used bioinformatics tools for the analysis to explore the possible constraints in micro-environment of each natural amino acid residue to be replaced with chlorine atom accommodation into GFPHS. In conclusion, our approaches are reliable and straightforward way to enhance the translation of chlorinated amino acids into proteins.
URI
http://hdl.handle.net/YU.REPOSITORY/27508http://dx.doi.org/10.1007/s12257-012-0066-6
ISSN
1226-8372
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생명공학부 > 생명공학부 > Articles
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