High performance liquid chromatography used for quality control of Achyranthis Radix

Title
High performance liquid chromatography used for quality control of Achyranthis Radix
Author(s)
손종근조병천[조병천]정수양[정수양]문동철[문동철]손건호[손건호]우미희[우미희]
Keywords
TANDEM MASS-SPECTROMETRY; QUANTIFICATION; BIDENTATAE
Issue Date
201208
Publisher
PHARMACEUTICAL SOC KOREA
Citation
ARCHIVES OF PHARMACAL RESEARCH, v.35, no.8, pp.1449 - 1455
Abstract
To establish a standard of quality control and to identify reliable Achyranthis Radix, three phytoecdysones including ecdysterone (1), 25R-inokosterone (2) and 25S-inokosterone (3) were determined by quantitative HPLC/UV analysis. Three phytoecdysones were separated with an YMC J'sphere ODS C-18 column (250 mm x 4.6 mm, 4 mu m) by isocratic elution using 0.1% formic acid in water and acetonitrile (85:15, v/v%) as the mobile phase. The flow rate was 1.0 mL/min and the UV detector wavelength was set at 245 nm. The standards were quantified by HPLC/UV from Achyranthes bidentata Blume and Achyranthes japonica Nakai, as well as Cyathula capitata Moq. and Cyathula officinalis Kuan, which are of a different genus but are comparative herbs. The method was successfully used in the analysis of Achyranthis Radix of different geographical origin or genera with relatively simple conditions and procedures, and the assay results were satisfactory for linearity, recovery, precision, accuracy, stability and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of eighteen A. bidentata Blume samples and ten A. japonica Nakai samples. The results indicate that the established HPLC/UV method is suitable for quantitation and pattern recognition analyses for quality evaluation of Achyranthis Radix.
URI
http://hdl.handle.net/YU.REPOSITORY/27506http://dx.doi.org/10.1007/s12272-012-0815-2
ISSN
0253-6269
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약학대학 > 약학부 > Articles
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