Intracerebroventricular injection of metformin induces anorexia in rats

Title
Intracerebroventricular injection of metformin induces anorexia in rats
Author(s)
김용운이창구최윤정박소영김종연원규장
Issue Date
201208
Citation
Diabetes and Metabolism Journal, v.36, no.4, pp.293 - 299
Abstract
Background: Metformin, an oral biguanide insulin-sensitizing agent, is well known to decrease appetite. Although there is evidence that metformin could affect the brain directly, the exact mechanism is not yet known. Methods: To evaluate whether metformin induces anorexia via the hypothalamus, various concentrations of metformin were injected into the lateral ventricle of rats through a chronically implanted catheter and food intake was measured for 24 hours. The hypothalamic neuropeptides associated with regulation of food intake were also analyzed following 1 hour of intracerebroventricular (ICV) injections of metformin. Results: An ICV injection of metformin decreased food intake in a dose-dependent manner in unrestrained conscious rats. Hypothalamic phosphorylated AMP-activated protein kinase (pAMPK) increased by 3 ��g with metformin treatment, but there was no further increase in pAMPK with increases in metformin dosage. The hypothalamic phosphorylated signal transducer and activator of transcription 3 (pSTAT3) increased by 3 ��g with metformin treatment, but, there was no further increase in pSTAT3 level following increases of metformin dosage. Hypothalamic proopiomelanocortin was elevated with metformin treatment, while neuropeptide Y was not significantly changed. Conclusion: Our results suggest that metformin induces anorexia via direct action in the hypothalamus and the increase in pSTAT3, at least in part, is involved in the process. However, hypothalamic pAMPK appears not to contribute to metformin-induced appetite reduction in normal rats. Further studies exploring new pathways connecting metformin and feeding regulation are needed. ? 2012 Korean Diabetes Association.
URI
http://hdl.handle.net/YU.REPOSITORY/27471http://dx.doi.org/10.4093/dmj.2012.36.4.293
ISSN
2233-6079
Appears in Collections:
의과대학 > 생리학교실 > Articles
의과대학 > 영상의학과학교실 > Articles
의과대학 > 내과학교실 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE