Quantitative determination of aceclofenac and its three major metabolites in rat plasma by HPLC-MS/MS

Title
Quantitative determination of aceclofenac and its three major metabolites in rat plasma by HPLC-MS/MS
Author(s)
강원구김은영[김은영]안병기노금한곽혜선[곽혜선]
Keywords
PERFORMANCE LIQUID-CHROMATOGRAPHY; DICLOFENAC
Issue Date
201209
Publisher
WILEY-V C H VERLAG GMBH
Citation
JOURNAL OF SEPARATION SCIENCE, v.35, no.17, pp.2219 - 2222
Abstract
We developed a method for the simultaneous quantification of aceclofenac and its three major metabolites in rat plasma. After protein precipitation with acetonitrile including flufenamic acid as an internal standard (IS), aceclofenac, diclofenac, 4'-hydroxyaceclofenac, 4'-hydroxydiclofenac, and the IS were chromatographed on a reverse-phase C18 analytical column. The isocratic mobile phase of acetonitrile/0.1% formic acid (aq; 9:1 [v/v]) was eluted at 0.3 mL/min. Quantification was performed on a triple-quadrupole mass spectrometer using electrospray ionization, and the ion transitions were monitored in selective reaction-monitoring mode. The coefficient of variation in the assay precision was less than 8%, and the accuracy was 92103%. This method was successfully used to measure the concentrations of aceclofenac and its three major metabolites in rat plasma following the oral administration of a single 20 mg/kg oral dose of aceclofenac.
URI
http://hdl.handle.net/YU.REPOSITORY/27359http://dx.doi.org/10.1002/jssc.201200367
ISSN
1615-9306
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약학대학 > 약학부 > Articles
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