Dual apoptotic DNA fragmentation system in the fly: Drep2 is a novel nuclease of which activity is inhibited by Drep3
- Dual apoptotic DNA fragmentation system in the fly: Drep2 is a novel nuclease of which activity is inhibited by Drep3
- 박현호; 박옥경
- CASPASE-ACTIVATED DNASE; DROSOPHILA-MELANOGASTER; DOMAIN; ICAD; AUTOIMMUNITY; PATHWAY; CANCER; DFF
- Issue Date
- ELSEVIER SCIENCE BV
- FEBS LETTERS, v.586, no.19, pp.3085 - 3089
- DNA fragmentation is the hallmark of apoptotic cells and mainly mediated by the DNA fragmentation factor DFF40(CAD)/DFF45(ICAD). DFF40 is a novel nuclease, whereas DFF45 is an inhibitor that can suppress the nuclease activity. Apoptotic DNA fragmentation in the fly is controlled by four DFF-related proteins, known as Drep1, 2, 3 and 4. However, the functions of Drep2 and Drep3 are totally unknown. Here, we found that Drep2 is a novel nuclease whose activity is inhibited by Drep3 through a tight interaction with the CIDE domain. Our results suggest that the fly has dual apoptotic DNA fragmentation systems: Drep1:Drep4 and Drep2:Drep3 complexes. Structured summary of protein interactions: Drep2 ODE and Drep-3 CIDE bind by blue native page (View interaction) Drep2 CIDE and Drep-3 CIDE bind by molecular sieving (View interaction) (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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