Production of astragaloside and flavones from adventitious root cultures of Astragalus membranaceus var. mongholicus

Title
Production of astragaloside and flavones from adventitious root cultures of Astragalus membranaceus var. mongholicus
Author(s)
배한홍Aye Aye Thwe[Aye Aye Thwe]Nguyen Thi Thanh Mai[ Nguyen Thi Thanh Mai]김예지[김예지]김연복[김연복]Md Romij Uddin[Md Romij Uddin]김영선[김영선]김행훈[김행훈]이미영[이미영]Xiaohua Li[Xiaohua Li]박상언[박상언]
Keywords
PLANT-CELL CULTURES; IN-VITRO; GROWTH; RADIX; IV; ISCHEMIA; IDENTIFICATION; CALYCOSIN; BARRIER; VIVO
Issue Date
201209
Publisher
SOUTHERN CROSS PUBL
Citation
PLANT OMICS, v.5, no.5, pp.466 - 470
Abstract
Many plants contain various secondary metabolites that have medicinal value. Their content varies across plant parts. We carried out in vitro adventitious root induction from leaf explants of Huang-qi (Astragalus membranaceus) using different nutrient media supplemented with various plant hormones. The level of astragaloside and flavones were analyzed from the adventitious roots of A. membranaceus grown under these different media conditions. Among the different media and plant hormones, Murashige and Skoog medium supplemented with 1.0 mg/L 1-naphthaleneacetic acid resulted in the greatest degree of adventitious root induction. The highest concentration of calycosin (11.7 mu g/g dry weight), calycosin-7-glu (13.3 mu g/g dry weight), and glucoraphanin (241.9 mu g/g dry weight) were observed in roots grown in half-strength Schenk & Hildebrandt mineral solution, half-strength Gamborg's B5 medium, and full-strength Gamborg's B5 media, respectively.
URI
http://hdl.handle.net/YU.REPOSITORY/27278
ISSN
1836-0661
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생명공학부 > 생명공학부 > Articles
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