Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene

Title
Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene
Author(s)
백광현차미정심상희김성홍[김성홍]김옥태[김옥태]이세원[이세원]권석윤[권석윤]
Keywords
1ST TOTAL-SYNTHESIS; TAXUS-BREVIFOLIA; TAXOL PRODUCTION; BACCATIN-III; PACIFIC YEW; PACLITAXEL; TAXA-4(5),11(12)-DIENE; MECHANISM; BIOSYNTHESIS; ARABIDOPSIS
Issue Date
201210
Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
Citation
BMB REPORTS, v.45, no.10, pp.589 - 594
Abstract
Paclitaxel is produced by various species of yew trees and has been extensively used to treat tumors. In our research, a taxadiene synthase (TS) gene from Taxus brevifolia was used to transform the roots of cultured ginseng (Panax ginseng C.A. Meyer) to produce taxadiene, the unique skeletal precursor to taxol. The TS gene was successfully introduced into the ginseng genome, and the de novo formation of taxadiene was identified by mass spectroscopy profiling. Without any change in phenotypes or growth difference in a TS-transgenic ginseng line, the transgenic TSS3-2 line accumulated 9.1 mu g taxadiene per gram cf dry weight In response to the treatment of methyl jasmonate for 3 or 6 days, the accumulation was 14.6 and 15.9 mu g per g of dry weight, respectively. This is the first report of the production of taxadiene by engineering ginseng roots with a taxadiene synthase gene. [BMB Reports 2012; 45(10): 589-594]
URI
http://hdl.handle.net/YU.REPOSITORY/27180http://dx.doi.org/10.5483/BMBRep.2012.45.10.085
ISSN
1976-6696
Appears in Collections:
생명공학부 > 생명공학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE