Simultaneous determination of zolazepam and tiletamine in dog plasma by liquid chromatography coupled to a tandem mass spectrometry

Title
Simultaneous determination of zolazepam and tiletamine in dog plasma by liquid chromatography coupled to a tandem mass spectrometry
Author(s)
강원구노금한김길수[김길수]안병기A, Pillippe[A, Pillippe]오태호[오태호]
Keywords
RAT PLASMA; TELAZOL(R)
Issue Date
201210
Publisher
WILEY-BLACKWELL
Citation
BIOMEDICAL CHROMATOGRAPHY, v.26, no.10, pp.1133 - 1136
Abstract
A mixture of tiletamine, a dissociative anesthetic, and zolazepam, a minor tranquilizer, has been widely used as an anesthetic or an immobilizing agent in a variety of animal species. However, interestingly, their pharmacokinetic behaviors have been published only in polar bears and pigs. In this study, we introduce a sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for determining the two drugs in dog plasma. After simple protein precipitation with acetonitrile including midazolam (internal standard), the analytes were chromatographed on a reversed-phase column with a mobile phase of 10?m m ammonium acetate aqueous solution and acetonitrile (1:4, v/v). The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This method was used to measure the concentrations of zolazepam and tiletamine in plasma after a single intramuscular 10?mg dose of each in beagle dogs. Copyright (c) 2012 John Wiley & Sons, Ltd.
URI
http://hdl.handle.net/YU.REPOSITORY/27157http://dx.doi.org/10.1002/bmc.2692
ISSN
0269-3879
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약학대학 > 약학부 > Articles
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