Anti-inflammatory effects of anthocyanins from black soybean seed coat on the keratinocytes and ischemia-reperfusion injury in rat skin flaps

Title
Anti-inflammatory effects of anthocyanins from black soybean seed coat on the keratinocytes and ischemia-reperfusion injury in rat skin flaps
Author(s)
허지안김혜정[김혜정]최태현[최태현]김상현[김상현]김석화[김석화]LIANJI XU[LIANJI XU]장기철[장기철]신성철[신성철]정종일[정종일]강다원[강다원]최재훈[최재훈]
Keywords
NF-KAPPA-B; TNF-ALPHA; ISCHEMIA/REPERFUSION INJURY; IN-VIVO; SURVIVAL; MODEL; PROTECTS; INHIBITION; MUSCLE; CELL
Issue Date
201210
Publisher
WILEY-BLACKWELL
Citation
MICROSURGERY, v.32, no.7, pp.563 - 570
Abstract
Ischemia-reperfusion injury is a phenomenon that occurs when tissues are subjected to ischemia for a variable period of time, and then reperfused. Inflammatory reaction has been implicated as one of the most important mechanism of ischemia-reperfusion injury. The purpose of this study was to evaluate the anti-inflammatory effects of anthocyanins from black soybean seed coat on keratinocytes in vitro and ischemia-reperfusion injury in vivo. We investigated the inhibition, by anthocyanins, of the expression of various inflammatory genes associated with ischemia-reperfusion injury in the tumor necrosis factor-alpha-treated (TNF-a) immortalized epidermal keratinocyte cell line (HaCaT). We also investigated the effects of anthocyanins on the survival of skin flaps after ischemia-reperfusion injury in the rats. According to Western blot analysis and a luciferase activity assay, anthocyanins inhibited TNF-a-induced intercellular adhesion molecule-1 and cyclooxygenase-2 (COX-2) levels through the NF-?B-dependent pathway. Administration of anthocyanins (50 and 100 mg/kg) significantly improved the flap area survival in the 10-hour ischemic model from 62% to 74.5% and 83%, respectively (P = 0.001). The related cytokines in skin flap also changed as the same pattern as in vitro. Our results indicate that anthocyanins from black soybean seed coat had anti-inflammatory effects on the HaCaT cell line and increase the survival of skin flaps through anti-inflammatory properties against ischemia-reperfusion injury. (c) 2012 Wiley Periodicals, Inc. Microsurgery, 2012.
URI
http://hdl.handle.net/YU.REPOSITORY/27148http://dx.doi.org/10.1002/micr.22019
ISSN
0738-1085
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의과대학 > 내과학교실 > Articles
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