Indole-3-acetaldehyde from Rhodococcus sp BFI 332 inhibits Escherichia coli O157:H7 biofilm formation

Title
Indole-3-acetaldehyde from Rhodococcus sp BFI 332 inhibits Escherichia coli O157:H7 biofilm formation
Author(s)
이진태조무환이진형김용규김창진[김창진]이재찬[이재찬]
Keywords
STAINLESS-STEEL; SP-NOV; EXPRESSION; O157-H7; GENE; EXOPOLYSACCHARIDE; CHEMOTAXIS; RESISTANCE; FASCIANS; O157H7
Issue Date
201211
Publisher
SPRINGER
Citation
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.96, no.4, pp.1071 - 1078
Abstract
Pathogenic biofilms have been associated with persistent infections due to their high resistance to antimicrobial agents. To identify nontoxic biofilm inhibitors for enterohemorrhagic Escherichia coli O157:H7, the spent media of a 4,104 Actinomycetes library was screened. The culture spent medium (1%, v/v) of plant pathogen Rhodococcus sp. BFI 332 markedly inhibited E. coli O157:H7 biofilm formation without affecting the growth of planktonic E. coli O157:H7 cells. Rhodococcus sp. BFI 332 produced significant amounts of indole-3-acetaldehyde and indole-3-acetic acid, and the former of which reduced E. coli O157:H7 biofilm formation. Global transcriptome analyses showed that indole-3-acetaldehyde most repressed two curli operons, csgBAC and csgDEFG, and induced tryptophanase (tnaAB) in E. coli O157:H7 biofilm cells. Electron microscopy showed that spent medium of Rhodococcus sp. BFI 332 and indole-3-acetaldehyde reduced curli production in E. coli O157:H7. The spent medium of Rhodococcus sp. BFI 332 also significantly reduced the biofilm formation of Staphylococcus aureus and Staphylococcus epidermidis. Overall, this study suggests that indole derivatives are present in the Actinomycetes strains and they can be used as biofilm inhibitors against pathogenic bacteria.
URI
http://hdl.handle.net/YU.REPOSITORY/27016http://dx.doi.org/10.1007/s00253-012-3881-y
ISSN
0175-7598
Appears in Collections:
공과대학 > 화학공학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE