Saucerneol F, a New Lignan Isolated from Saururus chinensis, Attenuates Degranulation via Phospholipase C gamma 1 Inhibition and Eicosanoid Generation by Suppressing MAP Kinases in Mast Cells

Title
Saucerneol F, a New Lignan Isolated from Saururus chinensis, Attenuates Degranulation via Phospholipase C gamma 1 Inhibition and Eicosanoid Generation by Suppressing MAP Kinases in Mast Cells
Author(s)
장현욱유예손종근
Keywords
C-KIT LIGAND; LEUKOTRIENE C-4 GENERATION; NF-KAPPA-B; TYROSINE KINASE; FACTOR-RECEPTOR; PROTEIN-KINASE; CROSS-LINKING; 5-LIPOXYGENASE; EXPRESSION; ACTIVATION
Issue Date
201211
Publisher
KOREAN SOC APPLIED PHARMACOLOGY
Citation
BIOMOLECULES & THERAPEUTICS, v.20, no.6, pp.526 - 531
Abstract
During our on-going studies to identify bioactive compounds in medicinal herbs, we found that saucerneol F (SF), a naturally occurring sesquilignan isolated from Saururus chinensis (S. chinensis), showed in vitro anti-inflammatory activity. In this study, we examined the effects of SF on the generation of 5-lipoxygenase (5-LO) dependent leukotriene C-4 (LTC4), cyclooxygenase-2 (COX-2) dependent prostaglandin D-2 (PGD(2)), and on phospholipase C gamma 1 (PLC gamma 1)-mediated degranulation in SCF-induced mouse bone marrow-derived mast cells (BMMCs). SF inhibited eicosanoid (PGD(2) and LTC4) generation and degranulation dose-dependently. To identify the molecular mechanisms underlying the inhibition of eicosanoid generation and degranulation by SF, we examined the effects of SF on the phosphorylation of PLC gamma 1, intracellular Ca2+ influx, the translocation of cytosolic phospholipase A(2) (cPLA(2)) and 5-LO, and on the phosphorylation of MAP kinases (MAPKs). SF was found to reduce intracellular Ca2+ influx by inhibiting PLC gamma 1 phosphorylation and suppressing the nuclear translocations of cPLA(2) and 5-LO via the phosphorylations of MAPKs, including extracellular signal-regulated protein kinase-1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), and p38. Taken together, these results suggest that SF may be useful for regulating mast cell-mediated inflammatory responses by inhibiting degranulation and eicosanoid generation.
URI
http://hdl.handle.net/YU.REPOSITORY/26992http://dx.doi.org/10.4062/biomolther.2012.20.6.526
ISSN
1976-9148
Appears in Collections:
약학대학 > 약학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE