Cell-free microRNAs in urine as diagnostic and prognostic biomarkers of bladder cancer

Title
Cell-free microRNAs in urine as diagnostic and prognostic biomarkers of bladder cancer
Author(s)
송필현윤석중[윤석중]정필두[정필두]김원태[김원태]김태환[김태환]이영숙[이영숙]최영현[최영현]김이삭[김이삭]문성권[문성권]김원재[김원재]
Keywords
EPITHELIAL-MESENCHYMAL TRANSITION; MIR-200 FAMILY; REPRESSORS ZEB1; TUMORS; EXPRESSION; MECHANISM; DEATH; SERUM; RNAS
Issue Date
201211
Publisher
SPANDIDOS PUBL LTD
Citation
INTERNATIONAL JOURNAL OF ONCOLOGY, v.41, no.5, pp.1871 - 1878
Abstract
miRNAs are small, non-coding RNAs that play important roles in various biological processes. The aims of our study were to investigate whether cell-free miRNAs can be measured in urine samples and might be an accurate biomarker of bladder cancer. Datasets of GSE20418 and GSE19717 were used for analysis, and two miRNAs, miR-145 and miR-200a, were selected for study. A total of 207 patients with primary transitional cell carcinoma of the urinary bladder and 144 healthy normal controls were enrolled. Using quantitative PCR, the levels of miR-145 and miR-200a in urine were measured and compared with the clinicopathological features of bladder cancer. According to our experiments, cell-free miRNAs were present in urine and were stable. Assessment of miR-145 levels was able to distinguish bladder cancer patients from non-cancer controls (77.8% sensitivity and 61.1% specificity for NMIBC, AUC 0.729; 84.1 and 61.1% for MIBC, respectively, AUC 0.790) and showed good correlation with grade (p=0.048). In addition, miR-200a was shown to be an independent predictor of NMIBC recurrence by multivariate analysis (OR 0.449, 95% CI 0.239-0.842, p=0.013). A higher risk of recurrence was observed among patients with a lower miR-200a level compared to patients with higher miR-200a levels (log-rank test, p=0.040). Urinary cell-free miRNAs show promise as noninvasive biomarkers for diagnosis and recurrence of bladder cancer.
URI
http://hdl.handle.net/YU.REPOSITORY/26976http://dx.doi.org/10.3892/ijo.2012.1622
ISSN
1019-6439
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의과대학 > 비뇨기과학교실 > Articles
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