Monodisperse Poly(lactide-co-glycolic acid)-Based Nanocarriers for Gene Transfection

Title
Monodisperse Poly(lactide-co-glycolic acid)-Based Nanocarriers for Gene Transfection
Author(s)
변정훈김희권[김희권]Jeffrey T. Roberts[Jeffrey T. Roberts]
Keywords
AEROSOL DRUG-DELIVERY; NONVIRAL VECTOR; NANOPARTICLES; RELEASE; CARRIERS; CELLS; MICROPARTICLES; MICELLES; SYSTEMS; SIZE
Issue Date
201211
Publisher
WILEY-V C H VERLAG GMBH
Citation
MACROMOLECULAR RAPID COMMUNICATIONS, v.33, no.21, pp.1840 - 1844
Abstract
This contribution describes a simple, aerosol-based method for fabricating monodisperse particles containing mixtures of poly(lactide-co-glycolic acid) [PLGA], protamine sulfate (Prot), and poly(l-lysine) [PLL] as nanocarriers for gene transfection. Aqueous solutions of PLGA, Prot, and PLL were collison-atomized, and the resulting aerosolized droplets were dried on the fly to form solid particles, which then were electrostatically size-classified into 50, 100, and 200 nm mobility diameter samples. Measurements of cell viability and transfection reveal that the fabricated nanocarriers have a lower cytotoxicity (>85% in cell viability) and a higher transfection efficiency [>8.7 x 10(5) in relative light units (RLU) mg(-1)] than does 25 kDa polyethyleneimine (approximate to 50% and 6.8 x 10(5) RLU mg(-1)).
URI
http://hdl.handle.net/YU.REPOSITORY/26968http://dx.doi.org/10.1002/marc.201200369
ISSN
1022-1336
Appears in Collections:
공과대학 > 기계공학부 > Articles
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