Dioscin sensitizes cells to TRAIL-induced apoptosis through downregulation of c-FLIP and Bcl-2

Title
Dioscin sensitizes cells to TRAIL-induced apoptosis through downregulation of c-FLIP and Bcl-2
Author(s)
이태진김용식[김용식]김은애박규건이성준김미선[김미선]손호용[손호용]
Keywords
CANCER CELLS; MEDIATED APOPTOSIS; OXIDATIVE STRESS; UP-REGULATION; CYCLE ARREST; GENERATION
Issue Date
201211
Publisher
SPANDIDOS PUBL LTD
Citation
ONCOLOGY REPORTS, v.28, no.5, pp.1910 - 1916
Abstract
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has received attention as a potential anticancer drug, because it induces apoptosis in a wide variety of cancer cells but not in most normal human cell types. Here, we showed that co-treatment with subtoxic doses of dioscin and TRAIL-induced apoptosis in Caki human renal cancer cells. Treatment of Caki cells with dioscin downregulated c-FLIPL and Bcl-2 proteins in a dose-dependent manner. Dioscin-induced decrease in c-FLIPL protein levels may be caused by the increased protein instability. We also found that dioscin induced downregulation of Bcl-2 at the transcriptional level. Pretreatment with NAC slightly inhibited the expression levels of c-FLIPL downregulated by the treatment of dioscin, suggesting that dioscin is partially dependent on the generation of ROS for downregulation of c-FLIPL. Taken together, the present study demonstrates that dioscin enhances TRAIL-induced apoptosis in human renal cancer cells by downregulation of c-FLIPL and Bcl-2.
URI
http://hdl.handle.net/YU.REPOSITORY/26959http://dx.doi.org/10.3892/or.2012.1962
ISSN
1021-335X
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의과대학 > 해부학교실 > Articles
의과대학 > 영상의학과학교실 > Articles
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