Microfluidic chip based chemiluminescence detection of L-phenylalanine in pharmaceutical and soft drinks

Title
Microfluidic chip based chemiluminescence detection of L-phenylalanine in pharmaceutical and soft drinks
Author(s)
당중덩Mohammad Kamruzzaman[Mohammad Kamruzzaman]Al-Mahmnur Alam[Al-Mahmnur Alam]Kyung Min Kim[Kyung Min Kim]Sang Hak Lee[Sang Hak Lee]Young Ho Kim[ Young Ho Kim]Gyu-Man Kim[Gyu-Man Kim]
Keywords
FLOW-INJECTION ANALYSIS; AMINO-ACIDS; LIQUID-CHROMATOGRAPHY; LUMINOL; SYSTEM; ELECTROPHORESIS; NANOPARTICLES; GLUCOSE; SENSOR; SERUM
Issue Date
201211
Publisher
ELSEVIER SCI LTD
Citation
FOOD CHEMISTRY, v.135, no.1, pp.57 - 62
Abstract
A sensitive chemiluminescence (CL) method on chip coupled with microfluidic system has been reported for the determination of L-phenylalanine (L-PA). A microfluidic chip device with the detection chamber capable of fast sensing light emitted from the luminol and hydrogen peroxide CL reaction catalyzed by copper sulphate was fabricated for the determination of L-PA. The microfluidic chip was fabricated by a soft-lithographic procedure using polydimethyl siloxane (PDMS). The fabricated PDMS microfluidic chip had four inlet microchannels for introducing the sample, chemiluminescent reagent, Cu(II), and oxidant, and a 500 mu m wide, 250 mu m deep, and 82 mm long microchannel. The detection was based on the enhancement effect of L-PA on the CL signals of luminol-H2O2-Cu2+ system in an alkaline medium. The CL intensity of the system was enhanced linearly with the concentration of L-PA in the range of 1.5 x 10(-9)-1.2 x 10(-7) mol L-1. The limit of detection was found to be 2.4 x 10(-10) mol L-1 with the relative standard deviation of 1.8%. The presented method offers a simple, rapid and easy to handle analytical technique in terms of sensitivity, dynamic range and low detection limit for the determination of L-PA in diet soft drinks and pharmaceutical injection samples. (c) 2012 Elsevier Ltd. All rights reserved,
URI
http://hdl.handle.net/YU.REPOSITORY/26952http://dx.doi.org/10.1016/j.foodchem.2012.04.062
ISSN
0308-8146
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공과대학 > 기계공학부 > Articles
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