Endocytic Pathway and Resistance to Cholesterol Depletion of Cholesterol Derived Cationic Lipids for Gene Delivery

Title
Endocytic Pathway and Resistance to Cholesterol Depletion of Cholesterol Derived Cationic Lipids for Gene Delivery
Author(s)
도경오배윤위김병길[김병길]박종원서영배[서영배]
Keywords
CAVEOLAE-MEDIATED ENDOCYTOSIS; TRANSFECTION EFFICIENCY; PROTEIN CORONA; INTRACELLULAR TRAFFICKING; DEPENDENT INTERNALIZATION; CELLULAR UPTAKE; OVARIAN-CANCER; IN-VITRO; LIPOPLEXES; CELLS
Issue Date
201212
Publisher
AMER CHEMICAL SOC
Citation
MOLECULAR PHARMACEUTICS, v.9, no.12, pp.3579 - 3585
Abstract
Cholesterol-based cationic lipids have been widely used because of biocompatibility and serum resistance. However, the reason for the effectiveness of cholesterol-based cationic lipids remains unclear. We compared the transfection route of CHOL-E, a cholesterol-based cationic lipid having an amine head and an ether linker, with that of DOTAP. The luciferase assay with chemical inhibitors and microscopic observation of pathway markers revealed that clathrin mediated endocytosis is the main pathway for CHOL-E and DOTAP. However, CHOL-E showed resistance to cholesterol depletion by methyl-beta-cyclodextrin. Furthermore, CHOL-E recovered the transfection efficiency of DOTAP from cholesterol depletion. These results suggested that superior transfection of CHOL-E might be partly derived from effects on the cell membrane.
URI
http://hdl.handle.net/YU.REPOSITORY/26879http://dx.doi.org/10.1021/mp300458h
ISSN
1543-8384
Appears in Collections:
의과대학 > 생리학교실 > Articles
의과대학 > 영상의학과학교실 > Articles
의과대학 > 내과학교실 > Articles
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