High-performance liquid chromatographic analysis for quantitation of marker compounds of Artemisia capillaris thunb.

Title
High-performance liquid chromatographic analysis for quantitation of marker compounds of Artemisia capillaris thunb.
Author(s)
손종근박경민이응김보라장해연황보경박동근지미연우미희[우미희]최재수[최재수]이제현[이제현]문동철[문동철]장현욱김재룡
Keywords
OXIDATIVE STRESS; ESSENTIAL OIL; CELLS; CONSTITUENTS; APOPTOSIS; INHIBITION; RATS; ACTIVATION; FRACTIONS; SCOPARONE
Issue Date
201212
Publisher
PHARMACEUTICAL SOC KOREA
Citation
ARCHIVES OF PHARMACAL RESEARCH, v.35, no.12, pp.2153 - 2162
Abstract
Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among 'Injinho' and 'Myeon-injin' and 'Haninjin' - A. capillaris collected in autumn, A. capillaris collected in spring and A. iwayomogi, which can be misused as 'Injinho' in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40A degrees C. Calibration and quantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40A degrees C. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognition analysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.
URI
http://hdl.handle.net/YU.REPOSITORY/26866http://dx.doi.org/10.1007/s12272-012-1213-5
ISSN
0253-6269
Appears in Collections:
약학대학 > 약학부 > Articles
의과대학 > 생화학.분자생물학교실 > Articles
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