Synthesis of NBD-Labeled DOTAP Analog to Track Intracellular Delivery of Liposome

Title
Synthesis of NBD-Labeled DOTAP Analog to Track Intracellular Delivery of Liposome
Author(s)
이태진도경오김병길[김병길]박종원서영배[서영배]
Keywords
CATIONIC LIPOPLEXES; TRANSFECTION; VESICLES
Issue Date
201301
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.23, no.1, pp.131 - 135
Abstract
A DOTAP analog labeled by NBD on the head group (DT-NBD) was designed and synthesized to label DOTAP liposome. The structure was confirmed by H-1 NMR and FAB-MS, and the fluorescence of the newly synthesized DT-NBD was observed by fluorescent microscopy. The transfection efficiency of DOTAP liposome containing DT-NBD was comparable to commonly used NBD PE in COS7 and MCF7 cells. Furthermore, the level of cellular uptake and fluorescent intensity of fluorescent liposome containing DT-NBD was higher than NBD PE. Therefore, the novel NBD-labeled DOTAP analog seems to be effectively used for investigation of the cellular interaction and transfection mechanism of DOTAP liposome.
URI
http://hdl.handle.net/YU.REPOSITORY/26858http://dx.doi.org/10.4014/jmb.1208.08083
ISSN
1017-7825
Appears in Collections:
의과대학 > 해부학교실 > Articles
의과대학 > 생리학교실 > Articles
의과대학 > 내과학교실 > Articles
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