Comparison of 12-lipoxygenase expression in vascular smooth muscle cells from old normotensive Wistar-Kyoto rats with spontaneously hypertensive rats

Title
Comparison of 12-lipoxygenase expression in vascular smooth muscle cells from old normotensive Wistar-Kyoto rats with spontaneously hypertensive rats
Author(s)
김희선김혜영정대원박호선이태윤
Keywords
AGE-DEPENDENT INCREASE; ANGIOTENSIN-II; NITRIC-OXIDE; ALZHEIMERS-DISEASE; MAMMALIAN LIPOXYGENASES; GUANYLATE-CYCLASE; OXIDATIVE STRESS; 12/15-LIPOXYGENASE; RECEPTOR; GROWTH
Issue Date
201301
Publisher
NATURE PUBLISHING GROUP
Citation
HYPERTENSION RESEARCH, v.36, no.1, pp.65 - 73
Abstract
Vascular aging and essential hypertension cause similar structural and molecular modifications in the vasculature. The 12-lipoxygenase (LO) pathway of arachidonic acid metabolism is linked to cell growth and the pathology of hypertension. Thus, elevated expression of 12-LO has been observed in vascular smooth muscle cells (VSMCs) from spontaneously hypertensive rats (SHR). In the present study, we investigated the differences in 12-LO expression and activity between VSMCs from old normotensive Wistar-Kyoto rats (old WKY, 90-week old) and SHR (13-week old). The protein and mRNA expression of basal or angiotensin II (Ang II)-induced 12-LO in old WKY VSMCs were higher than those in SHR VSMCs. The degradation rate of 12-LO mRNA in old WKY VSMCs was slower than that in SHR VSMCs. However, basal or Ang II-induced 12-LO mRNAs in both old WKY and SHR VSMCs decayed more rapidly than that in young WKY (13-week old) VSMCs. Higher expression of 12-LO in old WKY VSMCs than in SHR VSMCs was correlated with the expression level of Ang II subtype 1 receptor (AT(1)R). The reduced levels of nitric oxide (NO) in old WKY and SHR VSMCs compared with young WKY VSMCs were similar, and there was no significant difference in NO production between old WKY and SHR VSMCs transfected with 12-LO siRNA. In addition, in contrast to the proliferation of SHR VSMCs, the proliferation of old WKY VSMCs was not dependent on 12-LO activation. These results suggest that the potential role of 12-LO in normotensive aging vasculature may be different from that in SHR vasculature. Hypertension Research (2013) 36, 65-73; doi:10.1038/hr.2012.119; published online 9 August 2012
URI
http://hdl.handle.net/YU.REPOSITORY/26800http://dx.doi.org/10.1038/hr.2012.119
ISSN
0916-9636
Appears in Collections:
의과대학 > 미생물학교실 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE