In vitro and in vivo assessment of cytochrome P450-mediated herb-drug interaction of Ssang-hwa-tang

Title
In vitro and in vivo assessment of cytochrome P450-mediated herb-drug interaction of Ssang-hwa-tang
Author(s)
강원구이상윤[이상윤]이지윤[이지윤]권광일[권광일]오수진[오수진]마진열[마진열]김상겸[김상겸]
Keywords
TIME-DEPENDENT INHIBITION; MASS-SPECTROMETRY; METABOLISM; ENZYMES; INDUCTION; PRODUCTS; P450IIE1
Issue Date
201301
Publisher
ELSEVIER SCI LTD
Citation
FOOD CHEMISTRY, v.136, no.2, pp.450 - 457
Abstract
We have evaluated the herb-drug interaction potential of Ssang-hwa-tang (SHT) mediated by cytochrome P450 (CYP) inhibition/induction. Further, the effects of fermentation on the CYP-mediated herb-drug interaction potential were determined. SHT showed inhibitory activity toward CYP1A2, but not 2A6, 2B6, 2C9, 2C19, 2D6, 2E1, and 3A4 in human liver microsomes. The results of the enzyme kinetic study suggested that the SHT-induced CYP1A2 inhibition is mixed reversible inhibition. The hepatic CYP expression and activity in rats treated with SHT were examined. The expression/activity of CYP2E1 increased as a result of SHT extract treatment (P < 0.005 or P < 0.001, respectively), which raises the possibility that SHT may increase the toxicity of environmental toxicants through the elevation of CYP2E1-mediated metabolic activation. SHT fermentation using Lactobacillus fermentum or Lactobacillus gassed resulted in attenuation of the SHT-induced CYP1A2 inhibition, but not CYP2E1 induction, suggesting that changes in the chemical composition of SHT through fermentation can affect the inhibition of CYP1A2 activity. (C) 2012 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/26779http://dx.doi.org/10.1016/j.foodchem.2012.08.069
ISSN
0308-8146
Appears in Collections:
약학대학 > 약학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE