Antibiofilm activity of Streptomyces sp. BFI 230 and Kribbella sp BFI 1562 against Pseudomonas aeruginosa

Title
Antibiofilm activity of Streptomyces sp. BFI 230 and Kribbella sp BFI 1562 against Pseudomonas aeruginosa
Author(s)
이진태조무환김용규이진형김창진[김창진]이재찬[이재찬]주윤정[주윤정]
Keywords
BIOFILM FORMATION; SP-NOV; SIDEROPHORE BIOSYNTHESIS; SWARMING MOTILITY; VIRULENCE FACTORS; IRON; PAO1; IDENTIFICATION; SEQUENCE; DISPERSION
Issue Date
201212
Publisher
SPRINGER
Citation
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.96, no.6, pp.1607 - 1617
Abstract
Members of the actinomycetes family are a rich source of bioactive compounds including diverse antibiotics. This study sought to identify novel and non-toxic biofilm inhibitors from the actinomycetes library for reducing the biofilm formation of Pseudomonas aeruginosa PAO1. After the screening of 4104 actinomycetes strains, we found that the culture spent medium (1 %, v/v) of Streptomyces sp. BFI 230 and Kribbella sp. BFI 1562 inhibited P. aeruginosa biofilm formation by 90 % without affecting the growth of planktonic P. aeruginosa cells, while the spent media enhanced the swarming motility of P. aeruginosa. Global transcriptome analyses revealed that the spent medium of Streptomyces sp. BFI 230 induced expression of phenazine, pyoverdine, pyochelin synthesis genes, and iron uptake genes in P. aeruginosa. The addition of exogenous iron restored the biofilm formation and swarming motility of P. aeruginosa in the presence of the spent medium of Streptomyces sp. BFI 230, which suggests that the Streptomyces sp. BFI 230 strain interfered iron acquisition in P. aeruginosa. Experiments on solvent extraction, heat treatment, and proteinase K treatment suggested that hydrophilic compound(s), possibly extracellular peptides or proteins from Streptomyces sp. BFI 230 cause the biofilm reduction of P. aeruginosa. Together, this study indicates that actinomycetes strains have an ability to control the biofilm of P. aeruginosa.
URI
http://hdl.handle.net/YU.REPOSITORY/26771http://dx.doi.org/10.1007/s00253-012-4225-7
ISSN
0175-7598
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공과대학 > 화학공학부 > Articles
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