Metabolic profiling of millet (Panicum miliaceum) using gas chromatography-time-of-flight mass spectrometry (GC-TOFMS) for quality assessment

Title
Metabolic profiling of millet (Panicum miliaceum) using gas chromatography-time-of-flight mass spectrometry (GC-TOFMS) for quality assessment
Author(s)
김재광[김재광]박수연[박수연]여윤수[여윤수]조현석[조현석]김연복[김연복]배한홍박철호[박철호]이재헌[이재헌]박상언[박상언]
Keywords
SATIVA L. CULTIVARS; PHENOLIC-ACIDS; PREDICTION; RANKING
Issue Date
201301
Publisher
SOUTHERN CROSS PUBL
Citation
PLANT OMICS, v.6, no.1, pp.73 - 78
Abstract
Gas-chromatography coupled with time-of-flight mass spectrometry (GC-TOFMS) was used to determine the diversity among primary metabolites and phenolic acids in three varieties of millet (Panicum miliaceum L.). Three cultivars of millet seeds were germinated in a greenhouse, and the seedlings were transferred to the field and allowed them to grow for a period of 4 months. A total of 48 metabolites were identified from millet, including 43 primary metabolites and five phenolic acids. The metabolite profiles were subjected to principal component analysis (PCA) and partial least-squares discriminate analysis (PLS-DA) to evaluate the differences among varieties. PCA and PLS-DA fully distinguished the three varieties tested. Joongjuk millet was separated from the other varieties based on the high levels of metabolites, and appears to be a good candidate for future breeding programs because of its high phenolic acids levels. This GC-TOFMS-based metabolic profiling approach is a viable alternative method for evaluating food quality.
URI
http://hdl.handle.net/YU.REPOSITORY/26744
ISSN
1836-0661
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생명공학부 > 생명공학부 > Articles
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