Synthesis, characterization and in vitro studies of doxorubicin-loaded magnetic nanoparticles grafted to smart copolymers on A549 lung cancer cell line

Title
Synthesis, characterization and in vitro studies of doxorubicin-loaded magnetic nanoparticles grafted to smart copolymers on A549 lung cancer cell line
Author(s)
주상우Abolfazl Akbarzadeh[Abolfazl Akbarzadeh]Mohammad Samiei[Mohammad Samiei]Maryam Anzaby[Maryam Anzaby]요네스피로즈살라리Hamid Tayefi Nasrabadi[Hamid Tayefi Nasrabadi]Soodabeh Davaran[Soodabeh Davaran]
Keywords
IRON-OXIDE NANOPARTICLES; BLOCK-COPOLYMER; DRUG-DELIVERY; POLYMERIC MICELLES; BREAST-CANCER; RELEASE; HYPERTHERMIA; THERAPY; FE3O4
Issue Date
201212
Publisher
BIOMED CENTRAL LTD
Citation
JOURNAL OF NANOBIOTECHNOLOGY, v.10
Abstract
Background: The aim of present study was to develop the novel methods for chemical and physical modification of superparamagnetic iron oxide nanoparticles (SPIONs) with polymers via covalent bonding entrapment. These modified SPIONs were used for encapsulation of anticancer drug doxorubicin. Method: At first approach silane-grafted magnetic nanoparticles was prepared and used as a template for polymerization of the N-isopropylacrylamide (NIPAAm) and methacrylic acid (MAA) via radical polymerization. This temperature/pH-sensitive copolymer was used for preparation of DOX-loaded magnetic nanocomposites. At second approach Vinyltriethoxysilane-grafted magnetic nanoparticles were used as a template to polymerize PNIPAAm-MAA in 1, 4 dioxan and methylene-bis-acrylamide (BIS) was used as a cross-linking agent. Chemical composition and magnetic properties of Dox-loaded magnetic hydrogel nanocomposites were analyzed by FT-IR, XRD, and VSM. Results: The results demonstrate the feasibility of drug encapsulation of the magnetic nanoparticles with NIPAAm-MAA copolymer via covalent bonding. The key factors for the successful prepardtion of magnetic nanocomposites were the structure of copolymer (linear or cross-linked), concentration of copolymer and concentration of drug. The influence of pH and temperature on the release profile of doxorubicin was examined. The in vitro cytotoxicity test (MTT assay) of both magnetic DOx-loaded nanoparticles was examined. The in vitro tests showed that these systems are no toxicity and are biocompatible. Conclusion: IC50 of DOx-loaded Fe3O4 nanoparticles on A549 lung cancer cell line showed that systems could be useful in treatment of lung cancer.
URI
http://hdl.handle.net/YU.REPOSITORY/26687http://dx.doi.org/10.1186/1477-3155-10-46
ISSN
1477-3155
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공과대학 > 기계공학부 > Articles
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